AI Article Synopsis
- De novo addition of telomeric sequences occurs at broken chromosomes and is crucial during programmed DNA reorganization, especially in ciliated protozoa during macronuclear differentiation post-sexual reproduction.
- Small noncoding RNAs play a role in regulating these DNA-reorganization processes, which also require RNA templates from the parental macronucleus for excision and amplification.
- Research shows that microinjecting RNA templates with modified telomeres into the macronucleus results in altered telomeres, indicating that the addition of new telomeres relies on RNA transcripts containing telomeric repeats from the parent macronucleus.
Article Abstract
De novo addition of telomeric sequences can occur at broken chromosomes and must be well controlled, which is essential during programmed DNA reorganization processes. In ciliated protozoa an extreme form of DNA-reorganization is observed during macronuclear differentiation after sexual reproduction leading to the elimination of specific parts of the germline genome. Regulating these processes involves small noncoding RNAs, but in addition DNA-reordering, excision and amplification require RNA templates deriving from the parental macronucleus. We show that these putative RNA templates can carry telomeric repeats. Microinjection of RNA templates carrying modified telomeres into the developing macronucleus leads to modified telomeres in vegetative cells, providing strong evidence, that de novo addition of telomeres depends on a telomere-containing transcript from the parental macronucleus.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4993297 | PMC |
| http://dx.doi.org/10.1080/15476286.2015.1134414 | DOI Listing |
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