AI Article Synopsis
- - The study explores the molecular mechanisms behind nonhost disease resistance in plants, focusing on Nicotiana benthamiana and identifying key genes involved in this process using virus-induced gene silencing (VIGS).
- - Two important ribosomal protein genes, RPL12 and RPL19, were found to play a significant role in nonhost resistance; silencing these genes led to delayed hypersensitive responses and increased bacterial growth.
- - Additional findings indicate that both RPL12 and RPL19 function within the same pathway for nonhost resistance and also contribute slightly to basal resistance against virulent pathogens in both Nicotiana benthamiana and Arabidopsis.
Article Abstract
Characterizing the molecular mechanism involved in nonhost disease resistance is important to understand the adaptations of plant-pathogen interactions. In this study, virus-induced gene silencing (VIGS)-based forward genetics screen was utilized to identify genes involved in nonhost resistance in Nicotiana benthamiana. Genes encoding ribosomal proteins, RPL12 and RPL19, were identified in the screening. These genes when silenced in N. benthamiana caused a delay in nonhost bacteria induced hypersensitive response (HR) with concurrent increase in nonhost bacterial multiplication. Arabidopsis mutants of AtRPL12 and AtRPL19 also compromised nonhost resistance. The studies on NbRPL12 and NbRPL19 double silenced plants suggested that both RPL12 and RPL19 act in the same pathway to confer nonhost resistance. Our work suggests a role for RPL12 and RPL19 in nonhost disease resistance in N. benthamiana and Arabidopsis. In addition, we show that these genes also play a minor role in basal resistance against virulent pathogens.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4702080 | PMC |
| http://dx.doi.org/10.3389/fpls.2015.01192 | DOI Listing |
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