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Novel splicing variants of recepteur d'origine nantais (RON) tyrosine kinase involving exons 15-19 in lung cancer. | LitMetric

Novel splicing variants of recepteur d'origine nantais (RON) tyrosine kinase involving exons 15-19 in lung cancer.

Lung Cancer

Biomarkers Research Program, Riyadh Biochemistry Department, College of Science, King Saud University, 11451 Riyadh, Saudi Arabia; Prince Mutaib Chair for Biomarkers of Osteoporosis, Riyadh Biochemistry Department, College of Science, King Saud University, 11451 Riyadh, Saudi Arabia. Electronic address:

Published: February 2016

AI Article Synopsis

  • Altered expressions of receptor tyrosine kinases, particularly RON, are implicated in cancer growth and spread, yet therapeutic targeting is complicated by the existence of different isoforms with varying functions.
  • This study aimed to identify specific splicing variations in the RON gene to enhance understanding of how RON signaling operates in lung cancers by examining mRNA from various cancer cell lines.
  • The research found four novel RON transcript variants created by skipping certain exons, suggesting that tumors may generate dominant negative forms of RON that could inhibit normal signaling, raising questions about the effectiveness of therapies targeting the wild type RON.

Article Abstract

Background: Altered expressions of receptor tyrosine kinases drive the growth and metastasis of several cancers. RON is a single pass transmembrane receptor tyrosine kinase (RTK) shown to be aberrantly expressed in various cancer types. However, target validation and successful therapeutic targeting of RON in cancers is hampered by the co-existence of unknown number/types of isoforms, which are structurally similar but functionally diverse.

Objective: The objective of this study was to identify differential splicing in the C-terminal region of RON transcripts to better understand RON signaling in cancers. mRNA transcript sequence between exons 14 and 20 of RON was PCR amplified and sequenced using cDNA from 10 SCLC and 13 NSCLC cell lines. Specific exon deletions were identified by aligning sequencing chromatograms with reference RON cDNA sequence.

Results: We identified the presence of four unique transcript sequence variants of RON formed through skipping of exons 15-19, 16-19, 16-17 and 16. The transcript variants, except the one lacking exons 15-19, were found in more than one cell line. Several cell lines contained two to four of these uniquely spliced transcript variants. dbEST (Expressed Sequence Tags database) or other DNA sequence databases did not contain RON cDNA sequences corresponding to any of the above exon deletions indicating that all these transcript sequence alterations are novel.

Conclusions: Results of our study indicate common occurrence of different types of alternatively spliced transcripts of RON in lung cancer with potential to be translated into proteins lacking active kinase domain. Our findings suggest that tumors produce several dominant negative isoforms which probably inhibit ligand dependent RON signaling, and hence, raise important questions regarding the appropriateness of blocking wild type RON signaling for therapy. Further, presence of transcript variants and their isoform products may interfere with quantitative and functional analysis during target validation.

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Source
http://dx.doi.org/10.1016/j.lungcan.2015.12.002DOI Listing

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