AI Article Synopsis
- New potato varieties must be assessed for toxic glycoalkaloids (GAs) before being commercially released, as GAs can be harmful.
- This study focused on the connection between total glycoalkaloid (TGA) levels and the expression of specific genes (GAME, SGT1, SGT3) in potato tubers, using HPLC-MS and quantitative PCR for analysis.
- The research found that TGA content is linked to the expression of these genes, with promoter regions showing important cis-elements tied to stress responses and light, aiding our understanding of TGA regulation in potatoes.
Article Abstract
Before commercial release, new potato (Solanum tuberosum) varieties must be evaluated for content of toxic compounds such as glycoalkaloids (GAs), which are potent poisons. GA biosynthesis proceeds via the cholesterol pathway to α-chaconine and α-solanine. The goal of this study was to evaluate the relationship between total glycoalkaloid (TGA) content and the expression of GAME, SGT1, and SGT3 genes in potato tubers. TGA content was measured by HPLC-MS, and reverse transcription quantitative polymerase chain reactions were performed to determine the relative expression of GAME, SGT1, and SGT3 genes. We searched for cis-elements of the transcription start site using the PlantPAN database. There was a relationship between TGA content and the relative expression of GAME, SGT1, and SGT3 genes in potato tubers. Putative promoter regions showed the presence of several cis-elements related to biotic and abiotic stresses and light. These findings provide an important step toward understanding TGA regulation and variation in potato tubers.
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| http://dx.doi.org/10.1021/acs.jafc.5b05519 | DOI Listing |
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