AI Article Synopsis
- The study aimed to assess the stability of an in vitro biotransformation model using human intestinal bacteria to transform isoquercetin.
- It found that all models from different volunteers effectively converted isoquercetin into quercetin within 24 hours, with better efficiency noted as culture passages increased.
- The results indicate that the model is reliable and reproducible, making it suitable for researching chemical components in Chinese medicinal substances.
Article Abstract
Unlabelled: Objective: To investigate the stability of human intestinal bacterial biotransformation model using isoquercetin as the substrate of transformation. METHODs: The in vitro transformation model was established using the intestinal bacteria form different volunteers, or different passages of the same volunteer in accordance with the "biotransformation model of human intestinal bacteria and its standard operating procedures" to transform isoquercetin.
Results: Within 24 hours, all models established with the intestinal bacteria from different volunteers could transform isoquercetin to quercetin and the transformation efficiency was inclined to increase with the increase of the number of culture passages.
Conclusion: The intestinal bacterial model established in accordance with the "standard operating procedures" is stable and the results obtained with this model are reproducible, which demonstrats the suitability of this model for the investigation of the chemical constituents of Chinese medicinal materials.
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