Objective: To determine the bacterial colonisation after double-J stent use and the risk factors for bacteriuria linked to the stent.
Materials And Methods: A total of 102 patients (61 men and 41 women, mean age 47.5 ± 14.16) were examined. The stents were removed under aseptic conditions, and a urine culture was obtained before the removal of the stents. After the stents were removed, the upper, central and lower sections were separated, and washing water was sent through the stent.
Results: Bacterial colonisation was found in 29.4% (30 of 102) of the stents. The most frequently observed microorganisms were determined as staphylococcus, coagulase negative (8 of 30) and E. coli (5 of 30). The washing fluid used to clean the interior of the catheter produced pathogens in 8 patients (7.8%), and these pathogens were observed to be the same microorganisms that colonised the outside of the stent. There was no statistical difference between the patients with colonisation and those without in terms of age, gender, duration of stenting and reason for stent insertion.
Conclusions: Though stent colonisation does not always entail symptomatic urinary tract infections, as shown in our study, the pathogens in the urine culture are the same as those colonising the stent, confirming the reality that colonisation is the main factor in these events. Additionally, according to our study, significant colonisation may be found in the first 3 weeks, contrary to the literature, causing us to consider that urinary tract infections may develop even in the early period.
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http://dx.doi.org/10.4081/aiua.2015.4.291 | DOI Listing |
J Colloid Interface Sci
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State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China. Electronic address:
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UFZ-Helmholtz Centre for Environmental Research, Department of Soil Ecology, 06120 Halle (Saale), Germany.
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Centre for Nanomaterials and Biotechnology, Faculty of Science, University of Jan Evangelista Purkyně, Pasteurova 15, 400 96 Ústí nad Labem, Czech Republic.
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Center for Micro-Electro Mechanical Systems (CMEMS), Campus Azurém, University of Minho, 4800-058 Guimarães, Portugal.
Indwelling medical devices, such as urinary catheters, often experience bacterial colonization, forming biofilms that resist antibiotics and the host's immune defenses through quorum sensing (QS), a chemical communication system. This study explores the development of antimicrobial coatings by immobilizing acylase, a quorum-quenching enzyme, on sandblasted polydimethylsiloxane (PDMS) surfaces. PDMS, commonly used in medical devices, was sandblasted to increase its surface roughness, enhancing acylase attachment.
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