[Reference ranges of insulin, insulin like growth factor-1 and adrenocorticotropic hormone in ponies].

Objectives: The aim of this study was to validate a chemiluminescence immunometric assay using the IMMULITE 2000® for the determination of adrenocorticotropic hormone (ACTH), insulin and insulin-like growth factor-1 (IGF-1) from which reference ranges were calculated for ponies.

Material And Methods: Blood samples of 130 ponies aged 3-32 years were collected in the afternoon. The reference ranges were calculated according to the Guideline EP28-A3C of the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) from 2010.

Results: The determined intraday precision for insulin was 3.28%, for ACTH 3.35% and for IGF-1 1.84%. The interday precision (insulin: 3.45%; IGF-1: 2.89%; ACTH: 9.77%) was determined on three consecutive days, where the samples were stored at 4   °C. There was no significant loss of insulin activity nor of ACTH or IGF-1 concentration during this time. The reference ranges of insulin and IGF-1 (2.0-21.2 mU/l and 50.2-357.2 mU/l, respectively) were age independent, whereas for ACTH, an age-dependent reference range was established. Ponies aged 3-12 years had a significantly lower reference range (4.2-19.8 pg/ml) than ponies aged 13-32 years (5.0-22.6 pg/ml).

Conclusion: The method used is suitable for the determination of ACTH, IGF-1 and insulin in serum of ponies, but the lowest detection limit for insulin is 2.0 mU/l.

Clinical Relevance: The calculated reference ranges of insulin and ACTH are helpful for the diagnosis and clinical monitoring of equine metabolic syndrome and pituitary pars intermedia dysfunction (PPID).