This study demonstrated the protective efficiency of extracts of the Indian variety of Ophiocordyceps sinensis (=Cordyceps sinensis) (CSEs) in HT22 (murine hippocampal) cells under hypoxic conditions. Various parameters such as cell viability, reactive oxygen species, levels of endogenous antioxidants, inflammatory cytokines, transcription factors, and oxidation of macromolecules were analyzed. In addition, the radical scavenging abilities of hydroxyl radicals, nitric oxide, and superoxide radicals were also studied. Antioxidant compounds, ascorbic acid, hesperidin, and rutin were quantified by high-performance thin-layer chromatography. The information acquired from high-performance thin-layer chromatography profiling was subjected to principal component analysis for data clustering. Findings of this research revealed that ascorbic acid and rutin were highest in aqueous CSE, whereas the maximum amount of hesperidin was found in 25% alcoholic CSE. In vitro studies showed that all the CSEs protected HT22 cells well by upregulating the level of endogenous antioxidants and preventing the oxidation of lipids and proteins. These extracts also reduced the amount of hypoxia-induced inflammatory cytokines and transcription factors on par with the normoxic control with more or less equal protection in the cells under hypoxia, and indicated significant radical scavenging potential.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1615/intjmedmushrooms.v17.i9.30 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The fermentation of Cordyceps militaris polysaccharide influenced gut bacterial LPS structure formation and changed its antigenicity.
J Food Sci
December 2024
College of Food Science & Institute of Food Biotechnology, South China Agricultural University, Guangzhou, China.
Gut bacterial lipopolysaccharide (LPS) could be released into the circulatory system via the gut-liver axis and cause inflammatory immune response, while Cordyceps militaris polysaccharide (CMP40) has been reported to be effective in alleviating this inflammatory response. In this study, the effects of CMP40 gut fermentation on internal LPS structure formation and the subsequent immune response were explored. Results showed that CMP40 could change antigenicity of LPS of Vibrio parahaemolyticus, Salmonella enterica, and enterotoxigenic Escherichia coli, indicated by a reduced level of NO, IL-1β, IL-6, and TNF-α.
View Article and Find Full Text PDFFunctional Characterization and Toxicological Study of in Weaned Pigs.
Toxins (Basel)
November 2024
Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China.
(CM), a well-known parasitic fungus that grows on the larvae of , has a variety of pharmacological activities. However, little is known about its safe dosage for animals, including pigs. To explore its effect on intestinal health and evaluate its safe dosage, 30 weaned pigs were randomly allotted to five groups and fed with a basal diet supplemented with different doses of CM for 42 days.
View Article and Find Full Text PDFEvaluation of Lipid Changes During the Drying Process of by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS)-Based Lipidomics Technique.
J Fungi (Basel)
December 2024
State Key Laboratory of Plateau Ecology and Agriculture, Qinghai Academy of Animal and Veterinary Science, Qinghai University, Xining 810016, China.
Comprehensive analysis of the lipid content in samples is essential for optimizing their effective use. Understanding the lipid profile can significantly enhance the application of this valuable fungus across various fields, including nutrition and medicine. However, to date, there is limited knowledge regarding the effects of different drying methods on the quality of lipids present in .
View Article and Find Full Text PDFIn Vitro Response of Two Strains of to Six Chemical Pesticides.
J Fungi (Basel)
December 2024
College of Plant Protection, Shanxi Agricultural University, Taiyuan 030031, China.
To determine the compatibility of two new biocontrol fungi with common chemical pesticides, this study examined the effects of three insecticides, namely, avermectin, imidacloprid, and acetamiprid, and three fungicides, namely, chlorogenonil, boscalid, and kasugamycin, on the mycelial growth and spore germination of strains IF-1106 and IJ-tg19. The insecticidal effects of mixed insecticides or fungicides with good compatibility with IJ-tg19 against were tested. The results showed that the six chemical pesticides exerted different degrees of inhibition on the mycelial growth of both strains, with an obvious dose-dependent effect.
View Article and Find Full Text PDFA Ratiometric Fluorescence Method Based on PCN-224-DABA for the Detection of Se(IV) and Fe(III).
Biosensors (Basel)
December 2024
School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China.
In this study, 3,4-diaminobenzoic acid (DABA) was introduced into the porphyrin metal-organic framework (PCN-224) for the first time to prepare a ratiometric fluorescent probe (PCN-224-DABA) to quantitatively detect ferric iron (Fe(III)) and selenium (IV) (Se(IV)). The fluorescence attributed to the DABA of PCN-224-DABA at 345 nm can be selectively quenched by Fe(III) and Se(IV), but the fluorescence emission peak attributed to tetrakis (4-carboxyphenyl) porphyrin (TCPP) at 475 nm will not be disturbed. Therefore, the ratio of I/I with an excitation wavelength of 270 nm can be designed to determine Fe(III) and Se(IV).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!