A Gram-stain-positive, aerobic, rod-shaped, non-motile actinomycete strain, designated EGI 60007, was isolated from healthy roots of F. collected from Yili County, Xinjiang Province, north-west China. A polyphasic approach was applied to study the taxonomic position of the new isolate. The 16S rRNA gene sequence of strain EGI 60007 had highest similarities with members of the genus , including CAU 59 (96.98 % 16S rRNA gene sequence similarity), DSM 20419 (96.43 %) and THG-MD12 (96.14 %). The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain EGI 60007 clustered with members of the genus , and formed a distinct clade with CAU 59. The polar lipids detected for strain EGI 60007 were phosphatidylglycerol, diphosphatidylglycerol, one unidentified glycolipid and one unidentified lipid. The DNA G+C content was determined to be 63.3 mol%. The chemotaxonomic features of strain EGI 60007 showed typical characteristics of the genus , with MK-9 as the respiratory quinone, 2,4-diaminobutyric acid as the diamino acid in the peptidoglycan, and anteiso-C, anteiso-C and iso-C as the major fatty acids. The sugars of whole-cell hydrolysates were mainly mannose, rhamnose, ribose and glucose, and a minor amount of xylose. Based on the results of the phylogentic analysis supported by morphological, physiological, chemotaxonomic and other differentiating phenotypic characteristics, strain EGI 60007 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is EGI 60007 ( = CGMCC 1.15081 = KCTC 39112 = DSM 29943).
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1099/ijsem.0.000876 | DOI Listing |
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!