The upscaling process planning developed in this study primarily involved optimizing operating parameters, i.e., dilution ratios, during process designs. Minimal variable cost was used as an indicator for selecting the optimal combination of dilution ratios. The upper and lower mean confidence intervals obtained from the actual cultured cell density data were used as the final cell density stability indicator after the operating parameters or dilution ratios were selected. The process planning method and results were demonstrated through three case studies of batch culture simulation. They are (1) final objective cell densities were adjusted, (2) high and low light intensities were used for intermediate-scale cultures, and (3) the number of culture days was expressed as integers for the intermediate-scale culture.
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