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The role of nutritional vitamin D in CKD-MBD in children and adults with CKD, on dialysis and after kidney transplantation - a European consensus statement.
Nephrol Dial Transplant
January 2025
Department of Microbiology, Immunology and Transplantation; Nephrology and Renal Transplantation Research Group, KU Leuven, Leuven, Belgium.
Vitamin D deficiency is common in patients with chronic kidney disease (CKD) and associates with poor outcomes. Current clinical practice guidelines recommend supplementation with nutritional vitamin D as for the general population. However, recent large-scale, clinical trials in the general population failed to demonstrate a benefit of vitamin D supplementation on skeletal or non-skeletal outcomes, fueling a debate on the rationale for screening for and correcting vitamin D deficiency, both in non-CKD and CKD populations.
View Article and Find Full Text PDFEarly osteogenic differentiation of human dental stem cells by gelatin/calcium phosphate- Punica granatum nanocomposite scaffold.
BMC Biotechnol
January 2025
Department of Oral Science and Translation Research, College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, FL, 33314, USA.
Background: Tissue engineering for bone regeneration aims to heal severe bone injuries. This study aimed to prepare and assess the early osteogenic differentiation effects of a gelatin/calcium phosphate- Punica granatum nanocomposite scaffold on stem cells from human exfoliated deciduous (SHED) and human dental pulp stem cells (HDPSCs).
Methods: The electrospinning method was used to prepare a gelatin/calcium phosphate nanocomposite scaffold containing pomegranate (Punica granatum) extract.
Carboxylated Graphene: An Innovative Approach to Enhanced IgA-SARS-CoV-2 Electrochemical Biosensing.
Biosensors (Basel)
January 2025
LABEL-Laboratório de Bioeletrônica e Eletroanalítica, Central Analítica Multidisciplinar, Universidade Federal do Amazonas, Manaus 69067-005, Amazonas, Brazil.
Biosensors harness biological materials as receptors linked to transducers, enabling the capture and transformation of primary biorecognition signals into measurable outputs. This study presents a novel carboxylation method for synthesizing carboxylated graphene (CG) under acidic conditions, enhancing biosensing capabilities. The characterization of the CG was performed using scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), Raman spectroscopy, thermogravimetric analysis (TGA), and X-ray diffraction (XRD).
View Article and Find Full Text PDFWidespread occurrence and relevance of phosphate storage in foraminifera.
Nature
January 2025
SUGAR, X-star, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Japan.
Foraminifera are ubiquitous marine protists that intracellularly accumulate phosphate, an important macronutrient in marine ecosystems and in fertilizer potentially leaked into the ocean. Intracellular phosphate concentrations can be 100-1,000 times higher than in the surrounding water. Here we show that phosphate storage in foraminifera is widespread, from tidal flats to the deep sea.
View Article and Find Full Text PDFA novel bis-phosphonyl C-glycoside: the first synthesis of C-(1,6-deoxy-β-D-glucopyranosyl)dimethylphosphonate, a stable bisphosphonate to probe the mechanism of β-phosphoglucomutase.
Org Biomol Chem
January 2025
College of Pharmacy, Dalhousie University, 5968 College St., PO Box 15000, Halifax, Nova Scotia, B3H 4R2, Canada.
β-Phosphoglucomutase (β-PGM) catalyzes the interconversion of β-D-glucose-1-phosphate and β-D-glucose-6-phosphate sequentially utilizing a transient aspartyl-phospho enzyme and a β-D-glucose-1,6-bisphosphate intermediate. Herein, we report the first synthesis of the isosteric, cleavage resistant, phosphonate analogue C-(1,6-deoxy-β-D-glucopyranosyl)dimethylphosphonate, to aid in mechanistic and structural investigations of β-PGM and its phosphate transfer process. The introduction of the 'pseudo anomeric' phosphonate was accomplished through methylenephosphonate anion addition to gluconolactone, whilst the second phosphonate was installed at C-6 of the β-D-glucopyranosyl moiety using a Horner-Wadsworth-Emmons (HWE) reaction on the C-6 aldehyde.
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