AI Article Synopsis
- Scientists studied how the equine arteritis virus (EAV) affects cells and whether it causes cell death (apoptosis) in a specific cell line called RK13.
- They found that EAV made RK13 cells activate caspase-3, which helps trigger cell death, and that this activation depended on how much virus they used and how long the cells were infected.
- They also discovered that EAV affected other caspases (caspase-8, caspase-9, and caspase-12), showing that different types of cell lines respond differently to the virus, which can change how they survive or die.
Article Abstract
The modulation of the expression of caspases by viruses influences the cell survival of different cell types. Equine arteritis virus (EAV) induces apoptosis of BHK21 and Vero cell lines, but it is not known whether EAV induces apoptosis in RK13 cells, a common cell line routinely used in EAV diagnosis and research. In this study, we determined that caspase-3 expression was triggered after infection of RK13 cells with EAV in a time- and dose-dependent manner. We also detected caspase-8 and caspase-9 activation, indicating the stimulation of both extrinsic and intrinsic apoptosis pathways. Finally, we found caspase-12 activation, an indicator of endoplasmic reticulum stress-induced apoptosis. The variability observed in the apoptotic response in the different cell lines demonstrates that apoptosis depends on the distinctive sensitivity of each cell line used for investigation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.virusres.2015.12.010 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Electrolyzed Saline Prevents Virus Transmission in Dental Procedures: An In Vitro Study.
J Dent Res
December 2024
Department of Oral Medicine and Periodontology, University Clinical Centre Ljubljana, Ljubljana, Slovenia.
In dentistry, disinfection with antimicrobials is employed under different conditions and at different time points. During the COVID-19 pandemic, the use of disinfectant dental sprays was proposed, among other measures, to help prevent the transmission of infections during dental procedures that require highly effective antiseptics at particularly short contact times. The study aimed to evaluate the efficacy of electrolyzed saline (EOS) compared with other antiseptics in terms of the spread of enveloped and nonenveloped viruses by ultrasonic scaler (USS)-generated dental spray.
View Article and Find Full Text PDFProtective Role of Cepharanthine Against Equid Herpesvirus Type 8 Through AMPK and Nrf2/HO-1 Pathway Activation.
Viruses
November 2024
College of Agricultural Science and Engineering, Liaocheng University, Liaocheng 252000, China.
Equid herpesvirus type 8 (EqHV-8) is known to cause respiratory disease and miscarriage in horses and donkeys, which is a major problem for the equine farming industry. However, there are currently limited vaccines or drugs available to effectively treat EqHV-8 infection. Therefore, it is crucial to develop new antiviral approaches to prevent potential pandemics caused by EqHV-8.
View Article and Find Full Text PDFThe full transcription map of cottontail rabbit papillomavirus in tumor tissues.
PLoS Pathog
October 2024
Tumor Virus RNA Biology Section, The HIV Dynamics and Replication Program, NCI, NIH, Frederick, Maryland, United States of America.
Article Synopsis
- Cottontail rabbit papillomavirus (CRPV) serves as a key model for studying how certain viruses cause tumors, yet research has been hindered by a lack of comprehensive data on its transcriptome.
- The study developed a complete transcription map of CRPV using various advanced techniques, revealing distinct early and late RNA transcripts produced from five main promoters and two polyadenylation sites.
- It was found that CRPV undergoes significant alternative splicing, generating over 33 RNA isoforms that produce at least 12 viral proteins, thereby providing insights into how CRPV contributes to tumor development.
Identification of the Promoter Antisense Transcript Enhancing the Transcription of the Equine Herpesvirus-1 Immediate-Early Gene.
Viruses
July 2024
Laboratory of Comparative Pathology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Equine herpesvirus-1 (EHV-1) causes respiratory diseases, abortion, and encephalomyelitis in horses. The EHV-1 immediate-early (IE) protein, essential for viral replication, is transactivated by the binding of a multiprotein complex including the open reading frame 12 (ORF12) and some host factors to the IE promoter region. Promoter-associated non-coding RNAs (pancRNAs), which are transcribed from bidirectional promoters, regulate the transcription of neighboring genes in mammals and pathogens.
View Article and Find Full Text PDFImpact of equine herpesvirus-1 ORF15 (EUL45) on viral replication and neurovirulence.
Vet Microbiol
November 2024
Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan; Laboratory of Veterinary Microbiology, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Equine herpesvirus 1 (EHV-1) causes respiratory illness, fetal loss, perinatal mortality, and myeloencephalopathy. This study investigated ORF15's impact on virus infectivity and neurovirulence. The Ab4p neurovirulent strain of EHV1 was used as a backbone to create Ab4p attB, Ab4p∆ORF15, and Ab4p∆ORF15R chimeras via BAC DNA transfection into RK-13 cells.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!