In this paper, we study modularity of several functions which naturally arose in a recent paper of Lau and Zhou on open Gromov-Witten potentials of elliptic orbifolds. They derived a number of examples of indefinite theta functions, and we provide modular completions for several such functions which involve more complicated objects than ordinary modular forms. In particular, we give new closed formulae for special indefinite theta functions of type (1,2) in terms of products of mock modular forms. This formula is also of independent interest.
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http://dx.doi.org/10.1098/rsos.150310 | DOI Listing |
Physiol Plant
January 2025
Laboratory of Biochemistry, Institut Químic de Sarrià, Universitat Ramon Llull, Barcelona, Spain.
Photosynthetic microalgae are promising green cell factories for the sustainable production of high-value chemicals and biopharmaceuticals. The chloroplast organelle is being developed as a chassis for synthetic biology as it contains its own genome (the plastome) and some interesting advantages, such as high recombinant protein titers and a diverse and dynamic metabolism. However, chloroplast engineering is currently hampered by the lack of standardized cloning tools and Design-Build-Test-Learn workflows to ease genomic and metabolic engineering.
View Article and Find Full Text PDFACS Nano
January 2025
Department of Bioengineering, University of Washington, Seattle, Washington 98195-5061, United States.
The recent development of modular universal chimeric antigen receptor (CAR) T-cell platforms that use bifunctional adaptor intermediates to redirect engineered T-cell effector function has greatly expanded the capabilities of adoptive T-cell therapy, enabling safer and more comprehensive cancer treatment. However, universal CAR receptor systems rely on unstable transient recognition of tag-coupled intermediates for T-cell activation, and the array of targeting intermediates has been limited to antibodies and small molecules. Addressing these shortcomings, we engineered universal CAR T-cell receptors that can be covalently modified with synthetic biomaterials by accelerated SpyCatcher003-SpyTag003 chemistry for cancer-cell targeting.
View Article and Find Full Text PDFOrg Lett
January 2025
School of Pharmacy, Hangzhou Normal University, Hangzhou, Zhejiang 311121, P. R. China.
We demonstrate here an efficient and facile Ni-catalyzed electrochemical cross-electrophile thiolation approach for readily available alkyl alcohols with pyridyl thioesters. This C(sp)-S bond-forming modular strategy displays extensive substrate adaptability and good functional group tolerance, which allows the production of a range of alkyl sulfides with specific chemoselectivity. Furthermore, the potential applications of this methodology are illustrated by last-stage modification of bioactive molecules and sulfinylative cross-couplings.
View Article and Find Full Text PDFbioRxiv
January 2025
Chemical and Biological Engineering - Iowa State University, 618 Bissell Rd, Ames, IA 50011.
Proteins can be rapidly prototyped with cell-free expression (CFE) but in most cases there is a lack of probes or assays to measure their function directly in the cell lysate, thereby limiting the throughput of these screens. Increased throughput is needed to build standardized, sequence to function data sets to feed machine learning guided protein optimization. Herein, we describe the use of fluorescent single-walled carbon nanotubes (SWCNT) as effective probes for measuring protease activity directly in cell-free lysate.
View Article and Find Full Text PDFThe concentrations of individual proteins vary between cells, both developmentally and stochastically. The functional consequences of this variation remain largely unexplored due to limited experimental tools to manipulate the relationship of protein concentration to activity. Here, we introduce a genetically encoded tool based on a tunable amyloid that enables precise control of protein concentration thresholds in cells.
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