Aqueous two-phase system (ATPS) extractive bioconversion provides a technique which integrates bioconversion and purification into a single step process. Extractive bioconversion of gamma-cyclodextrin (γ-CD) from soluble starch with cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) enzyme derived from Bacillus cereus was evaluated using polyethylene glycol (PEG)/potassium phosphate based on ATPS. The optimum condition was attained in the ATPS constituted of 30.0% (w/w) PEG 3000 g/mol and 7.0% (w/w) potassium phosphate. A γ-CD concentration of 1.60 mg/mL with a 19% concentration ratio was recovered after 1 h bioconversion process. The γ-CD was mainly partitioned to the top phase (YT=81.88%), with CGTase partitioning in the salt-rich bottom phase (KCGTase=0.51). Repetitive batch processes of extractive bioconversion were successfully recycled three times, indicating that this is an environmental friendly and a cost saving technique for γ-CD production and purification.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jbiosc.2015.11.001 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Production and characterization of sophorolipid under yeast-mediated submerged fermentation utilizing Agro-industrial waste.
Curr Res Microb Sci
December 2024
Material Resource Efficiency Division, CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun 248005, Uttarakhand, India.
The challenges of pollution and agro-industrial waste management have led to the development of bioconversion techniques to transform these wastes into valuable products. This has increased the focus on the sustainable and cost-efficient production of biosurfactants from agro-industrial waste. Hence, the present study investigates the production of sophorolipid biosurfactants using the yeast strain IIPL32 under submerged fermentation, employing sugarcane bagasse hydrolysate-a renewable, low-cost agro-industrial waste as the feedstock.
View Article and Find Full Text PDFBacterial community profiling and identification of bacteria with lignin-degrading potential in different gut segments of African palm weevil larvae ().
Front Microbiol
January 2025
School of Science, Engineering and Environment, University of Salford, Salford, United Kingdom.
The microbiota within the guts of insects plays beneficial roles for their hosts, such as facilitating digestion and extracting energy from their diet. The African palm weevil (APW) lives within and feeds on the high lignin-containing trunk of palm trees; therefore, their guts could harbour a large community of lignin-degrading microbes. In this study, we aimed to explore the bacterial community within the gut of the APW larvae, specifically with respect to the potential for lignin degradation in various gut segments as a first step to determining the viability of mining bacterial lignin-degrading enzymes for the bioconversion of lignocellulosic biomass to biofuels and biomaterials.
View Article and Find Full Text PDFIn Vitro Bioaccessibility of Edible Seaweed Proteins from the Chilean Coast and Proteins from the Novel Seaweed-Derived Mycoprotein.
Molecules
January 2025
Department of Chemical Engineering, Biotechnology, and Materials, Centre for Biotechnology and Bioengineering (CeBiB), University of Chile, Santiago 8330111, Chile.
Seaweed biomass is globally underutilized as a source of proteins despite its nutritional potential, with much of its use focused on hydrocolloid extraction. This study evaluated the nutritional quality and digestibility of protein and amino acids from two brown seaweeds ( spp. and ), one green seaweed ( spp.
View Article and Find Full Text PDFBioconversion of Food and Green Waste into Valuable Compounds Using Solid-State Fermentation in Nonsterile Conditions.
Plants (Basel)
December 2024
Department of Molecular and Translational Medicine, University of Brescia, Viale Europa 11, 25123 Brescia, Italy.
Agro-industrial residues have transitions from being an environmental problem to being a cost-effective source of biopolymers and value-added chemicals. However, the efficient extraction of the desired products from these residues requires pretreatments. Fungal biorefinery is a fascinating approach for the biotransformation of raw materials into multiple products in a single batch.
View Article and Find Full Text PDFCharacterization of novel cold-active chitin deacetylase for green production of bioactive chitosan.
AMB Express
January 2025
Botany and Microbiology Department, Faculty of Science, Cairo University, Giza, 12613, Egypt.
A Novel cold-active chitin deacetylase from Shewanella psychrophila WP2 (SpsCDA) was overexpressed in Escherichia coli BL21 and employed for deacetylation of chitin to chitosan. The produced chitosan was characterized, and its antifungal activity was investigated against Fusarium oxysporum. The purified recombinant SpsCDA appeared as a single band on SDS-PAGE at approximately 60 kDa, and its specific activity was 92 U/mg.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!