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http://dx.doi.org/10.11622/smedj.2015191 | DOI Listing |
Front Cell Dev Biol
February 2024
Department of Molecular Biology, Pusan National University, Busan, Republic of Korea.
Ca is a key secondary messenger that modulates sperm motility by tuning flagellar movement in various species. The sperm-specific Ca channel, CatSper, is a primary Ca gate that is essential for male fertility in mammals. CatSper-mediated Ca signaling enables sperm to develop hyperactivated motility and fertilize the eggs in the female tract.
View Article and Find Full Text PDFFront Bioeng Biotechnol
November 2023
Research Center for Intelligent Sensing Systems, Zhejiang Laboratory, Hangzhou, China.
Single-nucleotide polymorphism (SNP) plays a key role in the carcinogenesis of the human genome, and understanding the intrinsic relationship between individual genetic variations and carcinogenesis lies heavily in the establishment of a precise and sensitive SNP detection platform. Given this, a powerful and reliable SNP detection platform is proposed by a flap endonuclease 1 (FEN 1)-driven DNA walker-like reaction coupling with a magnetic bead (MB)-based separation. A carboxyfluorescein (FAM)-labeled downstream probe (DP) was decorated on a streptavidin magnetic bead (SMB).
View Article and Find Full Text PDFBioengineering (Basel)
September 2023
Laboratory of Environmental and Life Sciences, University of Nova Gorica, Vipavska Cesta 13, P.O. Box 301, SI-5000 Nova Gorica, Slovenia.
Background: Protein complexes provide valuable biological information, but can be difficult to handle. Therefore, technical advancements designed to improve their manipulation are always useful.
Methods: We investigated the opportunity to exploit native agarose gels and the contact blot method for the transfer of native proteins to membranes as means for optimizing the conditions for obtaining stable complexes.
Sci Rep
March 2023
UniSQ College, University of Southern Queensland, Darling Heights, QLD, 4300, Australia.
The population growth and urbanization has caused an exponential increase in waste material. The proper disposal of waste is a challenging problem nowadays. The proper disposal site selection with typical sets and operators may not yield fruitful results.
View Article and Find Full Text PDFSaf Health Work
December 2022
Research Centre for Engineering and Agro-Food Processing, Council for Agricultural Research and Economics - Via Milano, 43 - 24047 Treviglio (BG), Italy.
Background: Agricultural handle equipment is present on all production areas' farms. They are handy and portable; however, excessive use can lead to acute traumas or accidental injuries. Repetitive movements, awkward postures, and hand-arm vibrations predispose them to pain and work-related musculoskeletal disorders.
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