We present a microfluidic chip in Polymethyl methacrylate (PMMA) for optical trapping of particles in an 80µm wide microchannel using two counterpropagating single-mode beams. The trapping fibers are separated from the sample fluid by 70µm thick polymer walls. We calculate the optical forces that act on particles flowing in the microchannel using wave optics in combination with non-sequential ray-tracing and further mathematical processing. Our results are compared with a theoretical model and the Mie theory. We use a novel fabrication process that consists of a premilling step and ultraprecision diamond tooling for the manufacturing of the molds and double-sided hot embossing for replication, resulting in a robust microfluidic chip for optical trapping. In a proof-of-concept demonstration, we show the trapping capabilities of the hot embossed chip by trapping spherical beads with a diameter of 6µm, 8µm and 10µm and use the power spectrum analysis of the trapped particle displacements to characterize the trap strength.
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http://dx.doi.org/10.1364/OE.23.030991 | DOI Listing |
J Med Life
November 2024
Biophysics and Cellular Biotechnology Department, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania.
Optical tweezers, which leverage the forces exerted by radiation pressure, have emerged as a pivotal technique for precisely manipulating and analyzing microscopic particles. Since Arthur Ashkin's ground-breaking work in the 1970s and the subsequent development of the single-beam optical trap in 1986, the capabilities of optical tweezers have expanded significantly, enabling the intricate manipulation of biological specimens at the micro- and nanoscale. This review elucidates the foundational principles of optical trapping and their extensive applications in the biomedical sciences.
View Article and Find Full Text PDFBiomech Model Mechanobiol
January 2025
CNR Istituto Officina Dei Materiali, Area Science Park Basovizza, S.S. 14, Km 163,5, 34149, Trieste, Italy.
The organization and dynamics of the spectrin-actin membrane cytoskeleton play a crucial role in determining the mechanical properties of red blood cells (RBC). RBC are subjected to various forces that induce deformation during blood microcirculation. Such forces also regulate membrane tension, leading to Piezo1 channel activation, which is functionally linked to RBC dehydration through calcium influx and subsequent activation of Gardos channels, ultimately resulting in variations in RBC volume.
View Article and Find Full Text PDFLab Chip
January 2025
Department of Chemistry, University of Victoria, Victoria, British Columbia, V8W 2Y2, Canada.
In the past decade, interest in nanoplasmonic structures has experienced significant growth, owing to rapid advancements in materials science and the evolution of novel nanofabrication techniques. The activities in the area are not only leading to remarkable progress in specific applications in photonics, but also permeating to and synergizing with other fields. This review delves into the symbiosis between nanoplasmonics and microfluidics, elucidating fundamental principles on nanophotonics centered on surface plasmon-polaritons, and key achievements arising from the intricate interplay between light and fluids at small scales.
View Article and Find Full Text PDFMicromachines (Basel)
December 2024
Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, MA 02155, USA.
The electric fields of tightly focused laser beams can be strong enough to apply appreciable force to microscopic objects, including biological entities such as cells, bacteria, and even viruses and biomolecules [...
View Article and Find Full Text PDFMaterials (Basel)
December 2024
Department of Physics, Gachon University, Seongnam-si 13120, Gyeonggi-do, Republic of Korea.
The rational design of heterojunction photocatalysts enabling fast transportation and efficient separation of photoexcited charge carriers is the key element in visible light-driven photocatalyst systems. Herein, we develop a unique Z-scheme heterojunction consisting of NiMoO microflowers (NMOF) and ZIF67, referred to as ZINM (composite), for the purpose of antibiotic degradation. ZIF67 was produced by a solution process, whereas NMOF was synthesized via coprecipitation with a glycine surfactant.
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