Objective: To examine the transfection of Homeobox A13 (HOXA13) on epithelial-mesenchymal transition (EMT) and the expression of bone morphogenetic protein-7 (BMP-7) induced by albumin-overload in human kidney tubular epithelial cells (HKCs).
Methods: The cultured HKCs were treated with 20 mg/mL human serum albumin (HSA) for 48 hours. Protein expression of cytokeratin (CK), vimentin and HOXA13 in the HKCs was assessed by Western blot. Protein expression of CK, vimentin, and BMP-7 was also detected in HKCs transfected with lipofectamine contained HOXA13 DNA.
Results: HSA induced EMT in HKCs, presented by decreased CK expression (P<0.01) and increased vimentin expression (P<0.01). The up-regulated expression of HOXA13 transfected by lipofectamine inhibited the level of EMT induced by HSA in HKCs (P<0.05). The decreased rate of BMP-7 protein expression induced by HSA was inhibited by over-expressed HOXA13 in HKCs (P<0.05).
Conclusions: Transfection of HOXA13 in HKCs could inhibit the degree of EMT induced by albumin-overload, possibly by increasing BMP-7 expression.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Unraveling MLL1-fusion leukemia: Epigenetic revelations from an iPS cell point mutation.
J Biol Chem
November 2024
Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, New York, United States. Electronic address:
Our understanding of acute leukemia pathology is heavily dependent on 11q23 chromosomal translocations involving the mixed lineage leukemia-1 (MLL1) gene, a key player in histone H3 lysine 4 (H3K4) methylation. These translocations result in MLL1-fusion (MLL1) proteins that are thought to drive leukemogenesis. However, the mechanism behind increased H3K4 trimethylation in MLL1-leukemic stem cells (MLL1-LSCs), following loss of the catalytic SET domain of MLL1 (known for H3K4 monomethylation and dimethylation) remains unclear.
View Article and Find Full Text PDFApplication of PLK1 and HOXA13 Gene Expression Levels in Urine in the Diagnosis of Non-muscle Invasive Bladder Cancer.
Biochem Genet
May 2024
Department of Clinical Biochemistry, School of Medicine, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences & Health Services, Yazd, Iran.
Bladder cancer is the most common urinary tract neoplasm, affecting many people annually. Current diagnostic and surveillance methods for bladder cancer are frequently invasive and lack sensitivity and specificity. This study aimed to develop an accurate and non-invasive urine-based gene expression assay, including fibroblast growth factor receptor 3 (FGFR3), homeobox A13 (HOXA13), and polo-like kinase 1 (PLK1), to diagnose non-muscle-invasive bladder cancer (NMIBC) at stages Ta and T1.
View Article and Find Full Text PDFHsa-microRNA-1249-3p/Homeobox A13 axis modulates the expression of β-catenin gene in human epithelial cells.
Sci Rep
December 2023
Department of Medical Sciences, University of Ferrara, 64/b, Fossato di Mortara Street, 44121, Ferrara, Italy.
Intercellular adhesion is a key function for epithelial cells. The fundamental mechanisms relying on epithelial cell adhesion have been partially uncovered. Hsa-microRNA-1249-3p (hsa-miR-1249-3p) plays a role in the epithelial mesenchymal transition in carcinoma cells, but its physiological function in epithelial cells is unknown.
View Article and Find Full Text PDFStructural characterization of SLYM-a 4th meningeal membrane.
Fluids Barriers CNS
December 2023
Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, 2200, Denmark.
Article Synopsis
- The meninges are traditionally classified into three layers: dura, arachnoid, and pia, but new evidence suggests they can be subdivided based on cellular characteristics.
- Researchers have identified a potential fourth layer called the Subarachnoid Lymphatic-like Membrane (SLYM) in certain mouse models, which covers the brain and assists with cerebrospinal fluid transport.
- Histological analyses revealed that SLYM interacts with existing layers and can alter the organization of the subarachnoid space, but caution is advised in interpreting these findings due to potential issues with tissue preparation techniques.
Article Synopsis
- The meninges consist of three known layers (dura, arachnoid, pia), but new research suggests they can be subdivided based on unique cellular features.
- Recent studies identified a potential fourth layer, called the Subarachnoid Lymphatic-like Membrane (SLYM), which plays a role in supporting cerebrospinal fluid transport around the brain and spinal cord.
- Researchers warn that the way these membranes are examined postmortem can lead to misinterpretations due to tissue shrinkage caused by fixatives.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!