AI Article Synopsis
- The paper presents a new luminescent iridium (III) complex that selectively detects G-quadruplex structures for rapid analysis of oligonucleotides and the VEGF165 protein.
- This biosensor enables label-free quantitative measurement of VEGF165 from cellular processes and investigates the interaction dynamics between aptamers and proteins using a microfluidic setup.
- The research highlights the effective monitoring of kinetic processes through hydrodynamic focusing and diffusive mixing, showcasing the potential of combining microfluidics with G-quadruplex probes for future studies in molecular interactions and recognition.
Article Abstract
In this paper, we rationally design a novel G-quadruplex-selective luminescent iridium (III) complex for rapid detection of oligonucleotide and VEGF165 in microfluidics. This new probe is applied as a convenient biosensor for label-free quantitative analysis of VEGF165 protein from cell metabolism, as well as for studying the kinetics of the aptamer-protein interaction combination with a microfluidic platform. As a result, we have successfully established a quantitative analysis of VEGF165 from cell metabolism. Furthermore, based on the principles of hydrodynamic focusing and diffusive mixing, different transient states during kinetics process were monitored and recorded. Thus, the combination of microfluidic technique and G-quadruplex luminescent probe will be potentially applied in the studies of intramolecular interactions and molecule recognition in the future.
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| http://dx.doi.org/10.1016/j.bios.2015.11.089 | DOI Listing |
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