Mass spectrometry detection of fraudulent use of cow whey in water buffalo, sheep, or goat Italian ricotta cheese.

Food Chem

Ministry of Agriculture Food and Forest Policies, Department of Central Inspectorate for Food and Feed Quality Protection and Fraud Repression, Central Laboratory of Rome, Via del Fornetto, 85, 00149 Rome, Italy. Electronic address:

Published: April 2016

AI Article Synopsis

  • - Ricotta cheese, a traditional Italian product, can be fraudulently made using cheaper cow whey instead of whey from sheep, goat, or buffalo.
  • - A new mass spectrometry method has been developed that identifies specific peptides from different whey sources, allowing for the detection of cow whey in ricotta cheese at levels as low as 0.5%.
  • - The method shows a strong correlation between the amount of cow whey and mass spectrometry results, making it a reliable tool for detecting and quantifying adulteration in ricotta cheese.

Article Abstract

Ricotta cheese is a typical Italian product, made with whey from various species, including cow, buffalo, sheep, and goat. Ricotta cheese nominally manufactured from the last three species may be fraudulently produced using the comparatively cheaper cow whey. Exposing such food frauds requires a reliable analytical method. Despite the extensive similarities shared by whey proteins of the four species, a mass spectrometry-based analytical method was developed that exploits three species-specific peptides derived from β-lactoglobulin and α-lactalbumin. This method can detect as little as 0.5% bovine whey in ricotta cheese from the other three species. Furthermore, a tight correlation was found (R(2)>0.99) between cow whey percentages and mass spectrometry measurements throughout the 1-50% range. Thus, this method can be used for forensic detection of ricotta cheese adulteration and, if properly validated, to provide quantitative evaluations.

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Source
http://dx.doi.org/10.1016/j.foodchem.2015.11.073DOI Listing

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