Simultaneous detection of single-nucleotide polymorphisms in a DNA bulge structure using a fluorine-modified bisbenzimide derivative.

Using 3,5-bis(trifluoromethyl)benzene-modified bisbenzimide H33258, bulged-out nucleobases on a DNA strand can be detected by the chemical shift change in (19)F NMR. Based on this technique, single-nucleotide polymorphisms on a DNA sequence can be analyzed simultaneously.