SpotCaliper: fast wavelet-based spot detection with accurate size estimation.

Motivation: SpotCaliper is a novel wavelet-based image-analysis software providing a fast automatic detection scheme for circular patterns (spots), combined with the precise estimation of their size. It is implemented as an ImageJ plugin with a friendly user interface. The user is allowed to edit the results by modifying the measurements (in a semi-automated way), extract data for further analysis. The fine tuning of the detections includes the possibility of adjusting or removing the original detections, as well as adding further spots.

Results: The main advantage of the software is its ability to capture the size of spots in a fast and accurate way.

Availability And Implementation: http://bigwww.epfl.ch/algorithms/spotcaliper/

Contact: zsuzsanna.puspoki@epfl.ch

Supplementary Information: Supplementary data are available at Bioinformatics online.