AI Article Synopsis
- Researchers used two-photon imaging and genetically encoded calcium indicators (GECIs) to study neuronal activity in adult common marmosets.
- The study implemented a method to enhance and control the expression of the GCaMP6f indicator, allowing for long-term observation (over 100 days) of neuronal activity.
- Findings included monitoring spontaneous activity across multiple neurons and cellular components, revealing how somatosensory cortex neurons respond to specific tactile stimuli and offering insights into cortical microcircuit organization.
Article Abstract
Two-photon imaging with genetically encoded calcium indicators (GECIs) enables long-term observation of neuronal activity in vivo. However, there are very few studies of GECIs in primates. Here, we report a method for long-term imaging of a GECI, GCaMP6f, expressed from adeno-associated virus vectors in cortical neurons of the adult common marmoset (Callithrix jacchus), a small New World primate. We used a tetracycline-inducible expression system to robustly amplify neuronal GCaMP6f expression and up- and downregulate it for more than 100 days. We succeeded in monitoring spontaneous activity not only from hundreds of neurons three-dimensionally distributed in layers 2 and 3 but also from single dendrites and axons in layer 1. Furthermore, we detected selective activities from somata, dendrites, and axons in the somatosensory cortex responding to specific tactile stimuli. Our results provide a way to investigate the organization and plasticity of cortical microcircuits at subcellular resolution in non-human primates.
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| http://dx.doi.org/10.1016/j.celrep.2015.10.050 | DOI Listing |
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