sp. nov., isolated from the rhizosphere of the mangrove L.

A Gram-stain-negative, motile, rod-shaped bacterium, designated MSSRFH1, was isolated from the rhizosphere of the mangrove, , in Pichavaram, Tamil Nadu, India. Phylogenetic analysis, based on the 16S rRNA gene sequence of MSSRFH1, indicated that it clustered in the genus and was most closely related to JCM18795 (96.7 % similarity). The 16S rRNA gene sequence similarity was < 96.5 % with other strains of species of the genus . The distinctiveness of strain MSSRFH1 was also shown by low similarities of its ( < 87 % similarity) and ( < 85 %) gene sequences with those of other members of the genus . Strain MSSRFH1 could tolerate NaCl concentrations of up to 30 % (w/v). The main fatty acids of MSSRFH1 included Cω7, C and Cω8. The polar lipids present included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified amino lipid and unidentified phospholipids. Comparison of BOX-PCR fingerprints indicated that MSSRFH1 showed a unique DNA profile and its genomic G+C content was 64 mol%. On the basis of the data presented, strain MSSRFH1 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is MSSRFH1 ( = KCTC 32998 = NBRC 110222).