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A combined proteomics and metabolomics approach to assess the effects of gold nanoparticles in vitro. | LitMetric

AI Article Synopsis

  • Omics technologies like proteomics and metabolomics have been underutilized in assessing the safety of nanomaterials, prompting a new integrated approach to study gold nanoparticles (AuNPs) of different sizes in Caco-2 cells.
  • In the study, exposure to 5 and 30 nm AuNPs for 72 hours led to the identification of 61 altered proteins and 35 annotated metabolites, revealing changes in various biological pathways.
  • The comprehensive analysis, supported by additional methods like immunochemistry and microscopy, enhances our understanding of cellular responses to nanomaterials and may help in identifying toxicity biomarkers and improving the safety of new nanomedicines.

Article Abstract

Omics technologies, such as proteomics or metabolomics, have to date been applied in the field of nanomaterial safety assessment to a limited extent. To address this dearth, we developed an integrated approach combining the two techniques to study the effects of two sizes, 5 and 30 nm, of gold nanoparticles (AuNPs) in Caco-2 cells. We observed differences in cells exposed for 72 h to each size of AuNPs: 61 responsive (up/down-regulated) proteins were identified and 35 metabolites in the cell extract were tentatively annotated. Several altered biological pathways were highlighted by integrating the obtained multi-omics data with bioinformatic tools. This provided a unique set of molecular information on the effects of nanomaterials at cellular level. This information was supported by complementary data obtained by immunochemistry, microscopic analysis, and multiplexed assays. A part from increasing our knowledge on how the cellular processes and pathways are affected by nanomaterials (NMs), these findings could be used to identify specific biomarkers of toxicity or to support the safe-by-design concept in the development of new nanomedicines.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4898143PMC
http://dx.doi.org/10.3109/17435390.2015.1121412DOI Listing

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