Background: According to the guideline of the United States center for disease control (CDC), the extracted teeth should be sterilized by autoclaving or storage in 10% formalin before using for educational or research purposes. The objective of this study was to evaluate the effect of this protocol on microhardness of dentin and enamel.
Material And Methods: Thirty extracted single-root teeth were used in this study. The crowns were resected, and the roots were longitudinally sectioned into two halves. The Vickers microhardness (VHN) of specimens was measured on polished canal dentin and buccal enamel surfaces. The crowns were randomly divided into three groups (n=10). Group 1 and 2 were sterilized using autoclave and formalin, respectively while group 3 (control) was stored in synthetic tissue fluid. The root halves were also randomly divided into 3 groups (n=20) which were treated as mentioned above for crown samples. Following sterilization, VHN of samples was measured again. ANOVA and paired samples t-tests were used to analyze the data.
Results: Autoclaving caused a significant reduction in microhardness of dentin (P <0.001, 12.04% decreases in VHN). However, there were no significant differences for before and after sterilization within other groups.
Conclusions: Based on the results of this study, the CDC protocol is recommended in studies related to enamel microhardness. However, Autoclaving is not an appropriate sterilization method in studies related to dentin microhardness. In these studies, two-week immersion in 10% formalin is recommended.
Key Words: Autoclave, CDC, extracted teeth, formalin, microhardness, sterilization.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4663054 | PMC |
| http://dx.doi.org/10.4317/jced.52280 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Exploring the pivotal variables of tongue diagnosis between patients with chronic kidney disease and health participants.
Front Big Data
January 2025
School of Chinese Medicine, China Medical University, Taichung, Taiwan.
Introduction: Chronic kidney disease (CKD) is a significant global health problem associated with high morbidity and mortality rates. Traditional Chinese Medicine (TCM) utilizes tongue diagnosis to differentiate symptoms and predict prognosis. This study examines the relationship between tongue characteristics and CKD severity using an automatic tongue diagnosis system (ATDS), which captures tongue images non-invasively to provide objective diagnostic information.
View Article and Find Full Text PDFAutomatic Vertical Root Fracture Detection on Intraoral Periapical Radiographs With Artificial Intelligence-Based Image Enhancement.
Dent Traumatol
January 2025
Department of Endodontology, Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv University, Tel Aviv, Israel.
Background/aim: To explore transfer learning (TL) techniques for enhancing vertical root fracture (VRF) diagnosis accuracy and to assess the impact of artificial intelligence (AI) on image enhancement for VRF detection on both extracted teeth images and intraoral images taken from patients.
Materials And Methods: A dataset of 378 intraoral periapical radiographs comprising 195 teeth with fractures and 183 teeth without fractures serving as controls was included. DenseNet, ConvNext, Inception121, and MobileNetV2 were employed with model fusion.
Biobanking of human teeth for stem cell storage: Preserving stem cells for future needs.
J Pak Med Assoc
January 2025
Department of Surgery, Aga Khan University Hospital, Karachi, Pakistan.
Human teeth serves as a potential reservoir for postembryonic mesenchymal dental stem cells. Researchers have identified and isolated seven types of dental stem cells from pulp and periodontal ligament tissues. These cells have a wide range of clinical applications across the fields of medicine and dentistry due to their increased proliferative nature.
View Article and Find Full Text PDFThree-dimensional quantitative temporomandibular joint changes in skeletal class I malocclusion treated with extraction and non-extraction protocols: a comparative study of fixed orthodontic appliances and clear aligners.
Prog Orthod
January 2025
Department of Orthodontics, School and Hospital of Stomatology, China Medical University, Shenyang, 110002, P.R. China.
Objective: This study aimed to evaluate the positional and morphological changes in the temporomandibular joint (TMJ) in adult patients with skeletal Class I malocclusion treated with fixed orthodontic appliances (FAs) and clear aligners (CAs), both with and without premolar extractions.
Methods: This retrospective study involved 120 adult patients divided into non-extraction and extraction groups, each further subdivided equally into those treated with FAs and CAs. Cone beam computed tomography (CBCT) was used to assess the TMJ measurements before (T0) and after treatment (T1).
Bonding performance of universal adhesive systems with dual-polymerising resin cements to various dental substrates: in vitro study.
BMC Oral Health
January 2025
Department of Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo, 1-5-45 Yushima, Bunkyo-Ku, Tokyo, 113-8549, Japan.
Background: Resin cements often require substrate-specific pretreatment. Recently, universal adhesive systems have been introduced, simplifying procedures by eliminating the need for multiple adhesives and offering options that do not require light curing. This study investigated the bonding performance of universal adhesive systems combined with dual-polymerising resin cements on enamel, dentin, zirconia, lithium disilicate ceramics (LDS), and resin blocks.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!