We hypothesized that addition of substances with antioxidant activity could decrease the concentrations of biomarkers of oxidative stress and inflammatory process, thus inhibiting nonalcoholic steatohepatitis development. We investigated the influence of α-lipoic acid (ALA) and garlic administration on the development of adverse changes in rabbit liver and serum under oxidative stress conditions induced with HFD from oxidized oils. We determined 8-hydroxy-2'-deoxyguanosine (8 OHdG) and malondialdehyde (MDA) in liver homogenates, total oxidant status (TOS), lipid peroxides (LOO) and tumor necrosis factor alpha (TNFα) in blood serum, and TNFα and IL-1α genes expression in liver. The results indicate that the intake of dietary ALA and garlic was significantly associated with decreases of 8 OHdG and MDA levels in rabbits' liver tissue as well as TOS and LOO levels in rabbits' serum. Similarly, TNFα and IL-1α gene expressions were suppressed due to ALA and garlic supplementation. The histopathological analysis confirmed that HFD results in liver disorder leading to steatosis. This adverse effect of HFD was ameliorated by the supplementation of ALA and garlic. The obtained results indicate a beneficial effect of ALA and garlic administration by reducing the oxidative stress intensity and the levels of some proinflammatory cytokines in rabbits fed HFD.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4655041 | PMC |
| http://dx.doi.org/10.1155/2015/827879 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Identification and Characterization of Bacterial Alliinase: Resource and Substrate Stereospecificity.
J Agric Food Chem
June 2024
State Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University, Qingdao 266237, P. R. China.
Article Synopsis
- * Researchers identified two bacterial cystathionine β-lyases with strong alliinase activity from rhizosphere isolates and explored their catalytic properties to enhance thiosulfinate production.
- * The findings highlight the ability of these bacterial enzymes to produce thiosulfinates with significant antimicrobial and antinematode effects, potentially benefiting crop protection and food preservation.
Content of amino acids and biogenic amines in stored meat as a result of a broiler diet supplemented with β-alanine and garlic extract.
Poult Sci
February 2024
Department of Animal Breeding, Institute of Animal Science, Warsaw University of Life Sciences-SGGW, 02-786 Warsaw, Poland.
Poultry meat is a highly esteemed product among consumers. However, the emphasis on increasing body weight has led to a rise in the proportion of rapidly shrinking fibers, adversely affecting the quality and shelf life of poultry meat. With a growing awareness of dietetics among consumers, there is an increasing challenge to produce chicken meat that is not only free of antibiotics but also beneficial for dietary and health reasons.
View Article and Find Full Text PDFDetermination of d- and l-Amino Acids in Garlic Foodstuffs by Liquid Chromatography-Tandem Mass Spectrometry.
Molecules
February 2023
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi-shi 274-8510, Japan.
Black garlic is currently attracting interest as a health food and constituent of commercial supplements; however, no data regarding the d-amino acids within black garlic have been reported. Therefore, the amino acid compositions of methanol extracts from fresh and black garlic were compared herein. We investigated the contents of the d- and l-forms of amino acids in commercial fresh, black, and freeze-dried garlic foodstuffs by liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a pre-column chiral derivatization reagent, succinimidyl 2-(3-((benzyloxy)carbonyl)-1-methyl-5-oxoimidazolidin-4-yl) acetate.
View Article and Find Full Text PDFPrevalence and Determinants of Complementary and Alternative Medicine (CAM) Use among Diabetes Patients in Southwestern Nigeria.
West Afr J Med
October 2020
Department of Medicine, General Hospital, Island, Lagos, Lagos State, Nigeria.
Introduction: The epidemic of diabetes mellitus (DM) in African countries demands a critical assessment and review of patients' management protocols and their self-care habits. The objective of this research was to evaluate the pattern of usage of CAM, its role in the management of diabetes and possible determinants of its use in patients with diabetes.
Methods: Subjects were recruited from six selected hospitals from four of the six States in South western, Nigeria.
Identification and antimicrobial activity evaluation of three peptides from laba garlic and the related mechanism.
Food Funct
August 2019
Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, P. R. China.
Laba garlic is a traditional Chinese processed garlic (Allium sativum L.) with multiple health benefits. This study was aimed at isolating and identifying three antimicrobial peptide fractions from laba garlic using Sephadex chromatography, pre-HPLC and LC-MS/MS and investigating the potential antimicrobial mechanism.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!