AI Article Synopsis
- Campylobacter jejuni has a polysaccharide capsule that is crucial for its classification using the Penner serotyping scheme, a method established in the 1980s.
- A new multiplex PCR technique has been developed that can identify all 47 known serotypes of C. jejuni, improving upon the earlier system.
- This new PCR method successfully determined capsule types in 98% of 996 clinical isolates tested from Thailand, Cambodia, and Nepal.
Article Abstract
Campylobacter jejuni produces a polysaccharide capsule that is the major determinant of the Penner serotyping scheme. This passive slide agglutination typing system was developed in the early 1980's and was recognized for over two decades as the gold standard for C. jejuni typing. A preliminary multiplex PCR technique covering 17 serotypes was previously developed in order to replace this classic serotyping scheme. Here we report the completion of the multiplex PCR technology that is able to identify all the 47 Penner serotypes types known for C. jejuni. The number of capsule types represented within the 47 serotypes is 35. We have applied this method to a collection of 996 clinical isolates from Thailand, Cambodia and Nepal and were able to successfully determine capsule types of 98% of these.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4668122 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0144349 | PLOS |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!