Serial femtosecond crystallography (SFX) allows structures to be determined with minimal radiation damage. However, phasing native crystals in SFX is not very common. Here, the structure determination of native lysozyme from single-wavelength anomalous diffraction (SAD) by utilizing the anomalous signal of sulfur and chlorine at a wavelength of 1.77 Å is successfully demonstrated. This sulfur SAD method can be applied to a wide range of proteins, which will improve the determination of native crystal structures.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4667287 | PMC |
| http://dx.doi.org/10.1107/S139900471501857X | DOI Listing |
Publication Analysis
Top Keywords
serial femtosecond
8
femtosecond crystallography
8
determination native
8
native
4
native sulfur/chlorine
4
sulfur/chlorine sad
4
sad phasing
4
phasing serial
4
crystallography serial
4
crystallography sfx
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!