The aim of the present study was to investigate the diagnostic value of cell cycle-related genes in oral squamous cell carcinoma (OSCC) by examining the expression of the following genes in 77 OSCC tissues by quantitative polymerase chain reaction: Cyclin genes (, , and ), cyclin-dependent kinase (CDK) genes (, and ), CDK inhibitor genes (, , and ), and integrin and associated genes that we previously reported (, , and ). The expression ratios of 66 combinations of the 11 cell cycle-related genes were analyzed to examine their associations with major clinical events using Mann-Whitney U and log-rank tests. Three expression ratios (/, / and /) showed associations on univariate analyses and their diagnostic value was re-analyzed with integrin gene expression biomarkers (/ and /) using the Cox proportional hazards model and Kaplan-Meier estimates. Lymph node metastasis occurred in >90% of double-positive cases (high-/ and high-/) irrespective of tumor size (P<0.0001). Primary site recurrence was found in >30% of double-positive cases (high-/ and high-/) with tumors >20 mm (P=0.003). Triple-positive (high-/, high-/ and high-/) was associated with distant metastasis (P<0.0001), but not with other clinical parameters. Disease-specific death occurred in 55% of double-positive cases (high-/ and high-/) (P<0.0001) and a positive surgical margin was a significant factor for fatality in these cases. Reliable prediction of locoregional and hematogenous dissemination risks in OSCC using the four CDK and integrin gene expression ratios is a promising biomarker system. Clinical use of these parameters may improve the control rate with the use of new therapeutic strategies.
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| http://dx.doi.org/10.3892/mco.2015.578 | DOI Listing |
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