AI Article Synopsis
- The chapter discusses somatic embryogenesis (SE) in Araucaria angustifolia, a native and endangered conifer from southern Brazil.
- It outlines the SE process, which involves several stages: induction, proliferation, pre-maturation, and maturation of embryogenic cultures made up of pro-embryogenic masses (PEMs).
- Double-staining techniques are highlighted as effective methods for evaluating the somatic structures at various developmental stages, and the chapter provides detailed protocols for A. angustifolia SE.
Article Abstract
This chapter deals with the features of somatic embryogenesis (SE) in Araucaria angustifolia, an endangered and native conifer from south Brazil. In this species SE includes the induction and proliferation of embryogenic cultures composed of pro-embryogenic masses (PEMs), which precede somatic embryos development. A. angustifolia SE model encompasses induction, proliferation, pre-maturation, and maturation steps. Double-staining with acetocarmine and Evan's blue is useful to evaluate the embryonic somatic structures. In this chapter we describe A. angustifolia SE protocols and analyzes morphological features in the different SE developmental stages.
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| http://dx.doi.org/10.1007/978-1-4939-3061-6_24 | DOI Listing |
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