AI Article Synopsis
- Researchers studied lactic acid bacteria (LAB) in Tibetan chickens to find safe strains that could deliver foreign proteins for vaccines.
- They identified a strain called Lactobacillus salivarius TCMM17, which strongly adheres to chicken gut cells and shows no harmful effects or resistance to antibiotics.
- By inserting a specific gene into TCMM17, they created a modified strain that can maintain this gene over generations and display the related protein on its surface, paving the way for new oral vaccines against bird diseases.
Article Abstract
To obtain adhesive and safe lactic acid bacteria (LAB) strains for expressing heterologous antigens, we screened LAB inhabitants in intestine of Tibetan chickens by analyzing their adhesion and safety properties and the selected LAB was engineered to express heterologous antigen (UTEpi C-A) based on chromosomal integration strategy. We demonstrated that a new Lactobacillu salivarius TCMM17 strain is strongly adhesive to chicken intestinal epithelial cells, contains no endogenous plasmids, is susceptible to tested antimicrobials, and shows no toxicities. In order to examine the potential of TCMM17 strain as heterogenous antigen delivering vehicle, we introduced a UTEpi C-A expression cassette in its chromosome by constructing a non-replicative plasmid (pORI280-UUTEpi C-AD). The recombinant TCMM17 strain (∆TCMM17) stably was found to keep the gene cassette through 50 generations, and successfully displayed EpiC encoded by the cassette on its surface. This work provides a universal platform for development of novel oral vaccines and expression of further antigens of avian pathogens.
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| http://dx.doi.org/10.1080/09168451.2015.1101330 | DOI Listing |
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a Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, ''985 Project'' Science Innovative Platform for Resource and Environment Protection of Southwestern, Key Laboratory of Bio-resources and Eco-environment , School of Life Science, Sichuan University, Ministry of Education , Chengdu , China.
Article Synopsis
- Researchers studied lactic acid bacteria (LAB) in Tibetan chickens to find safe strains that could deliver foreign proteins for vaccines.
- They identified a strain called Lactobacillus salivarius TCMM17, which strongly adheres to chicken gut cells and shows no harmful effects or resistance to antibiotics.
- By inserting a specific gene into TCMM17, they created a modified strain that can maintain this gene over generations and display the related protein on its surface, paving the way for new oral vaccines against bird diseases.
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