sp. nov. isolated from cave soil.

Int J Syst Evol Microbiol

National Key Laboratory of Agricultural Microbiology,College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, PRChina.

Published: February 2016

A Gram-stain-positive, spore-forming, motile, strictly aerobic, rod-shaped bacterium, designated strain L5, was isolated from soil of Tenglong cave, China. 16S rRNA gene sequence analysis showed that strain L5 was related most closely to MA001 (96.5 %) (the highest 16S rRNA gene sequence similarity), BT080 (96.4 %) and ZLD-8 (96.2 %). The DNA G+C content of strain L5 was 45.6 mol%. The major menaquinone was MK-7. The major fatty acids were iso-C, anteiso-C and iso-C, and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The diagnostic diamino acid in the cell-wall peptidoglycan was -diaminopimelic acid. In addition, strain L5 had different characteristics compared with the other strains such as pink colony colour, low growth temperature and low nutrient requirement. The results indicate that strain L5 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is L5 ( = KCTC 33637 = CCTCC AB 2015055).

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Source
http://dx.doi.org/10.1099/ijsem.0.000794DOI Listing

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