Intraparenchymal Stereotaxic Delivery of rAAV and Special Considerations in Vector Handling.

Methods Mol Biol

Department of Translational Science & Molecular Medicine, Michigan State University, 333 Bostwick Ave NE, Grand Rapids, MI, 49503-2532, USA.

Published: October 2016

Stereotaxic surgery enables precise and consistent microinjections to discrete neural nuclei. Using stereotaxic surgery to deliver viral vectors is a powerful tool that provides the ability to manipulate gene expression in specific regions, or even specific cell types in the brain. Here, we describe the proper handling and stereotaxic delivery of recombinant adeno-associated virus to various neuroanatomical structures of the rodent brain.

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http://dx.doi.org/10.1007/978-1-4939-3271-9_14DOI Listing

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