Purpose: The purpose of vitro study was to assessment of the bacterial contamination on daily twice-used toothbrushes in different conditions after 1-month and 3 months.
Materials And Methods: Forty children aged between 6 and 12 years were selected for this study. Toothbrushes and toothpaste were distributed among those children. Among those children, 20 children brushed their teeth for 1-month and 20 brushed their teeth for 3 months twice a day. Among those, 10 were kept in same brush box with a family member and 10 were kept in separate brush box after the use. Toothbrush from every child was recollected to investigate the contamination of microorganisms. Head of the toothbrushes transferred to a tube containing 10 ml of tryptone soy broth. This was followed by vortex mixing for 1 min to dislodge suspected adherent bacteria. The bacterial suspension was serially diluted to obtain dilution factors of up to 10(-3). 1 ml each of the dilution factors was obtained using a sterile pipette and plated on plate count agar. Petri dishes containing agar media were incubated and examined using a compound microscope.
Results: There was high mean difference between 1-month and 3 months, twice a day used toothbrush those kept with family members and those kept separate.
Conclusions: Toothbrush should keep in a separate box. Moreover, toothbrush should to be change after 3-4 weeks.
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http://dx.doi.org/10.4103/0976-9668.166072 | DOI Listing |
Br Dent J
January 2025
School of Clinical Dentistry, University of Sheffield, Sheffield, United Kingdom.
Introduction Supervised toothbrushing programmes (STPs) in nurseries and schools are effective at reducing inequalities in caries when targeted to areas of dental disease. Recent changes to government education and health policy have increased interest in STPs in England. This study aimed to establish the current level of provision of STPs in England, describe changes over time, understand associations with predictor variables, and summarise key barriers and facilitators to their implementation.
View Article and Find Full Text PDFNPJ Biofilms Microbiomes
January 2025
Department of Archaeogenetics, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany.
The genus Streptococcus is highly diverse and a core member of the primate oral microbiome. Streptococcus species are grouped into at least eight phylogenetically-supported clades, five of which are found almost exclusively in the oral cavity. We explored the dominant Streptococcus phylogenetic clades in samples from multiple oral sites and from ancient and modern-day humans and non-human primates and found that clade dominance is conserved across human oral sites, with most Streptococcus reads assigned to species falling in the Sanguinis or Mitis clades.
View Article and Find Full Text PDFCureus
December 2024
Medical Affairs, Dr. Reddy's Laboratories Ltd., Hyderabad, IND.
Background Toothbrush manufacturers commonly use bristle materials such as nylon, polybutylene terephthalate, polypropylene, polyethylene terephthalate, boar hair, bamboo, carbon fiber, silicone, polylactic acid, or their modifications such as Curen. Nylon filaments have long been demonstrated to be durable and are widely used, but not much is known regarding the performance of Curen filaments compared to nylon filaments. This in vitro study compared the stiffness, abrasion potential, abrasion resistance, and bristle surface changes of Curen and nylon filaments.
View Article and Find Full Text PDFDent J (Basel)
November 2024
Department of Medical, Oral and Biotechnological Sciences, University G. d'Annunzio, 66100 Chieti, Italy.
Little has been written in the literature about the clinical comparison between the single use of chlorhexidine (CHX) and its combination with cetylpyridinium chloride (CPC). The purpose of this study is to compare the clinical effectiveness of two at-home antiseptic regimens. : Healthy subjects scheduled for dental surgery were enrolled.
View Article and Find Full Text PDFMol Genet Metab Rep
December 2024
Scientific Laboratory of Molecular Genetics, Rīga Stradiņš University, LV-1007 Rīga, Latvia.
Background: Phenylketonuria (PKU) is an autosomal recessive inherited disorder of phenylalanine (Phe) metabolism that results from a deficiency of phenylalanine hydroxylase (PAH). Patients with PKU rely on amino acid mixtures and low-protein diets, which often exhibit an acidic nature and pose various challenges to oral health. The objective of the study was to evaluate oral care habits of PKU patients in Latvia and the impact of the recommendations developed on improving oral care.
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