AI Article Synopsis
- - Serum or plasma samples are mixed with a stable isotope labeled standard, allowing for precise measurement of pancreatic polypeptide (PP) and its truncated version, PP3-36.
- - Both forms of the peptides are extracted using anti-PP antibodies linked to magnetic beads and then separated from the beads with an acidic solution.
- - The separated peptides are analyzed using liquid chromatography and tandem mass spectrometry, employing electrospray ionization and a method called multiple reaction monitoring for accurate detection.
Article Abstract
Aliquots of serum or plasma samples are combined with stable isotope labeled internal standard. Pancreatic polypeptide (PP) and its truncated variant PP3-36 are enriched by incubation with anti-PP antibody conjugated to magnetic beads. Peptides are eluted from beads in acidic buffer and the samples analyzed using liquid chromatography coupled with tandem mass spectrometry. Instrumental analysis of PP and PP3-36 is performed using electrospray ionization ESI in positive ion mode and multiple reaction monitoring (MRM) acquisition.
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| http://dx.doi.org/10.1007/978-1-4939-3182-8_21 | DOI Listing |
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