Rapid imaging of surgical breast excisions using direct temporal sampling two photon fluorescent lifetime imaging.

Biomed Opt Express

Department of Electrical Engineering and Computer Science and Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA, USA.

Published: November 2015

Two photon fluorescent lifetime imaging is a modality that enables depth-sectioned, molecularly-specific imaging of cells and tissue using intrinsic contrast. However, clinical applications have not been well explored due to low imaging speed and limited field of view, which make evaluating large pathology samples extremely challenging. To address these limitations, we have developed direct temporal sampling two photon fluorescent lifetime imaging (DTS-FLIM), a method which enables a several order of magnitude increase in imaging speed by capturing an entire lifetime decay in a single fluorescent excitation. We use this greatly increased speed to perform a preliminary study using gigapixel-scale imaging of human breast pathology surgical specimens.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4646541PMC
http://dx.doi.org/10.1364/BOE.6.004317DOI Listing

Publication Analysis

Top Keywords

photon fluorescent
12
fluorescent lifetime
12
lifetime imaging
12
direct temporal
8
temporal sampling
8
sampling photon
8
imaging speed
8
imaging
7
rapid imaging
4
imaging surgical
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!