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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4658201 | PMC |
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0143900 | PLOS |
Sci Rep
December 2024
Department of Chemical Engineering, Polytechnic School, University of São Paulo, Av. Prof. Luciano Gualberto, Travessa 3, n. 380., São Paulo, SP, CEP 05508-900, Brazil.
16S ribosomal nucleic acid (16S rRNA) analysis allows to specifically target the metabolically active members of microbial communities. The stability of the ratios between target genes in the workflow, which is essential for the bioprocess-relevance of the data derived from this analysis, was investigated using synthetic mock communities constructed by mixing purified 16S rRNA from Bacillus subtilis (Bs), Staphylococcus aureus (Sa), Pseudomonas aeruginosa (Pa), Klebsiella pneumoniae (Kp) and Burkholderia cepacia (Bc) in different proportions. The RT reaction yielded one copy of cDNA per rRNA molecule for Pa, Bc and Sa but only 2/3 of the expected cDNA from 16S rRNAs of Bs and Kp.
View Article and Find Full Text PDFJ Exp Orthop
January 2025
Department of Trauma and Orthopaedics, Institute for Locomotion, Sainte-Marguerite Hospital Aix-Marseille University Marseille France.
Purpose: Asymmetric anterior closing-wedge high tibial osteotomy (ACWHTO) allows correction of both excessive posterior tibial slope (PTS) and varus deformity. However, the complexity of this surgery requires a high degree of accuracy, which is less likely to be achieved with standard instrumentations. This study aimed to determine the accuracy of 3D patient-specific cutting guides (PSCGs) to provide the accurate planned correction in the frontal and sagittal planes.
View Article and Find Full Text PDFBMC Med Educ
December 2024
Department of Pediatrics and Child Health, School of Medicine, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia.
Background: Electrocardiography (ECG) interpretation competency is vital to ensure the timely initiation of life-saving treatment for emergent ECG conditions. This competency has not been well-studied among pediatric and child health residents. Hence, the study aimed to determine the competency in ECG interpretation and its predictors among residents at the National University of Ethiopia.
View Article and Find Full Text PDFBMC Med Res Methodol
December 2024
Institute for Health Services Research and Clinical Epidemiology, Philipps-Universität Marburg, Karl-von-Frisch-Strasse 4, Marburg, 35043, Germany.
Background: Health services research often relies on secondary data, necessitating quality checks for completeness, validity, and potential errors before use. Various methods address implausible data, including data elimination, statistical estimation, or value substitution from the same or another dataset. This study presents an internal validation process of a secondary dataset used to investigate hospital compliance with minimum caseload requirements (MCR) in Germany.
View Article and Find Full Text PDFJ Headache Pain
December 2024
Department of Neuromedicine and Movement Science, Norwegian University of Science and Technology, Edvard Griegs gate, Trondheim, Norway.
Background: We have previously shown headache disorders to be prevalent in in the adult general population of Morocco, especially migraine (30.8%) and headache on ≥ 15 days/month (H15+; 10.5%).
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