AI Article Synopsis
- Staphylococcus aureus is a dangerous bacteria that can cause hard-to-treat chronic wounds and may survive antibiotic treatments due to persister cells.
- When treated with ciprofloxacin, survival rates of S. aureus varied based on the antibiotic concentration, with optimal effectiveness at 1 µg/mL, significantly reducing bacterial survival.
- The study revealed that this variation was linked to prophage induction, which helped eliminate the persister cells, suggesting that these findings could inform better quinolone treatment strategies.
Article Abstract
Staphylococcus aureus is a notorious pathogen with a propensity to cause chronic, non-healing wounds. Bacterial persisters have been implicated in the recalcitrance of S. aureus infections, and this motivated us to examine the persistence of S. aureus to ciprofloxacin, a quinolone antibiotic. Upon treatment of exponential phase S. aureus with ciprofloxacin, we observed that survival was a non-monotonic function of ciprofloxacin concentration. Maximal killing occurred at 1 µg/mL ciprofloxacin, which corresponded to survival that was up to ~40-fold lower than that obtained with concentrations ≥ 5 µg/mL. Investigation of this phenomenon revealed that the non-monotonic response was associated with prophage induction, which facilitated killing of S. aureus persisters. Elimination of prophage induction with tetracycline was found to prevent cell lysis and persister killing. We anticipate that these findings may be useful for the design of quinolone treatments.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4695809 | PMC |
| http://dx.doi.org/10.3390/ph8040778 | DOI Listing |
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