Extending the durability of plant resistance genes towards fungal pathogens is a major challenge. We identified and investigated the relationship between two avirulence genes of Leptosphaeria maculans, AvrLm3 and AvrLm4-7. When an isolate possesses both genes, the Rlm3-mediated resistance of oilseed rape (Brassica napus) is not expressed due to the presence of AvrLm4-7 but virulent isolates toward Rlm7 recover the AvrLm3 phenotype. Combining genetic and genomic approaches (genetic mapping, RNA-seq, BAC (bacterial artificial chromosome) clone sequencing and de novo assembly) we cloned AvrLm3, a telomeric avirulence gene of L. maculans. AvrLm3 is located in a gap of the L. maculans reference genome assembly, is surrounded by repeated elements, encodes for a small secreted cysteine-rich protein and is highly expressed at early infection stages. Complementation and silencing assays validated the masking effect of AvrLm4-7 on AvrLm3 recognition by Rlm3 and we showed that the presence of AvrLm4-7 does not impede AvrLm3 expression in planta. Y2H assays suggest the absence of physical interaction between the two avirulence proteins. This unusual interaction is the basis for field experiments aiming to evaluate strategies that increase Rlm7 durability.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/nph.13736 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Avirulence depletion assay: Combining R gene-mediated selection with bulk sequencing for rapid avirulence gene identification in wheat powdery mildew.
PLoS Pathog
January 2025
Chair of Phytopathology, TUM School of Life Sciences, Technical University of Munich, Freising, Germany.
Wheat production is threatened by multiple fungal pathogens, such as the wheat powdery mildew fungus (Blumeria graminis f. sp. tritici, Bgt).
View Article and Find Full Text PDFComparative Genome Analysis of Piscine : Virulence-Associated Metabolic Pathways.
Microorganisms
December 2024
Department of Biochemistry, Faculty of Science, Kasetsart University, 50 Ngam Wong Wan, Chatuchak, Bangkok 10900, Thailand.
Vibriosis caused by is a major problem in aquatic animals, particularly brown marble groupers (). biotype I has recently been isolated and classified into subgroups SUKU_G1, SUKU_G2, and SUKU_G3 according to the different types of virulence genes. In a previous study, we have shown that biotype I strains were classified into three subgroups according to the different types of virulence genes, which exhibited different phenotypes in terms of growth rate and virulence.
View Article and Find Full Text PDFIdentification and Genome Sequencing of Novel Virulent Strains of pv. Causing Rice Bacterial Blight in Zhejiang, China.
Pathogens
December 2024
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MOA of China and Zhejiang Province, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Science, Hangzhou 310000, China.
pv. () is the causative agent of rice bacterial blight (RBB), resulting in substantial harvest losses and posing a challenge to maintaining a stable global supply. In this study, strains isolated from Shaoxing, Quzhou, and Taizhou, where RBB occurred most frequently in Zhejiang Province in 2019, were selected as the subjects of research.
View Article and Find Full Text PDFComparative Transcriptome Analysis Highlights the Role of in the Honeydew Production of Virulent Brown Planthoppers ( Stål) to Resistant Rice Variety.
Insects
December 2024
College of Life Sciences, Xinyang Normal University, Xinyang 464000, China.
Brown planthoppers (BPHs, Stål) are a major threat to rice cultivation in Asia, necessitating the development of pest-resistant varieties for effective management. However, the adaptability of BPHs has resulted in the development of virulent populations, such as biotype Y BPHs, which exhibit significant virulence against the rice variety YHY15 that harbors the resistance gene . The various response mechanisms of BPH populations to resistant rice varieties are critical yet underexplored.
View Article and Find Full Text PDFSurveys of genotypes in breeding stations and commercial rice fields in Arkansas, Louisiana, and Puerto Rico from 2017 to 2019.
Plant Dis
January 2025
USDA-ARS SEA, Dale Bumpers National Rice Research Center, Stuttgart, Arkansas, United States;
Major resistance (R) gene mediated resistance to rice blast fungus Magnaporthe oryzae is often overcome by the fungus due to the occurrences of new races with altered corresponding avirulence (AVR) genes. In this study, blast diseased rice tissue samples were collected from breeding stations and commercial rice fields in Arkansas, Louisiana, and Puerto Rico during 2017-2019 to determine the efficacy of major R genes, Pi-ta, Pik, Pizt, Pi9, and Pi33. A total of 185 blast isolates were isolated from the diseased tissue samples to examine the existence of AVR genes AVR-Pita1, AVR-Pib, AVR-Pik, AVR-Pizt, AVR-Pi9 and ACE1.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!