Zscan10 is dispensable for maintenance of pluripotency in mouse embryonic stem cells.

Biochem Biophys Res Commun

Laboratory for Pluripotent Stem Cell Studies, RIKEN Center for Developmental Biology (CDB), 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan; Laboratory for Development and Regenerative Medicine, Kobe University Graduate School of Medicine, 7-5-1 Kusunokicho, Chuo-ku, Kobe 650-0017, Japan; JST, CREST, Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan; Department of Pluripotent Stem Cell Biology, Institute of Molecular Embryology and Genetics (IMEG), 2-2-1 Honjo, Chuo-ku, Kumamoto 860-0811, Japan. Electronic address:

Published: December 2015

Zinc finger and SCAN domain-containing 10 (Zscan10, also known as Zfp206) encodes a transcription factor that has been reported to be involved in the maintenance of pluripotency in mouse embryonic stem (ES) cells. Here we generated inducible knockout ES cells for Zscan10 using the Cre-loxP system and analyzed its function. We succeeded in establishing Zscan10-null ES cells and confirmed their pluripotency by the generation of chimeric embryos. Our results clearly indicate that Zscan10 is dispensable for the ability of self-renewal and differentiation in ES cells.

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http://dx.doi.org/10.1016/j.bbrc.2015.11.039DOI Listing

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