Many efforts have been made to produce artificial materials with biomimetic properties for applications in binding assays. Among these efforts, the technique of molecular imprinting has received much attention because of the high selectivity obtainable for molecules of interest, robustness of the produced polymers, simple and short synthesis, and excellent cost efficiency. In this review, progress in the field of molecularly imprinted sorbent assays is discussed-with a focus on work conducted from 2005 to date.
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http://dx.doi.org/10.1007/s00216-015-9137-9 | DOI Listing |
J Fluoresc
January 2025
Central Research Laboratory, Kastamonu University, 37200, Kastamonu, Turkey.
Fluorescence characterization of halophilic archaeal C50 carotenoid-bacterioruberin extracts was investigated using UV/Vis and steady-state fluorescence spectrophotometry in solvents with different polarity. Different extracts showed maximum absorption and fluorescence wavelengths between 369-536 nm and 540-569 nm. Stokes' shifts varied between 50-79 nm depending on the solvent.
View Article and Find Full Text PDFBiotechnol Bioeng
January 2025
Bioprocess Research and Development (BRD), WuXi Biologics, Shanghai, China.
Serving as a dedicated process analytical technology (PAT) tool for biomass monitoring and control, the capacitance probe, or dielectric spectroscopy, is showing great potential in robust pharmaceutical manufacturing, especially with the growing interest in integrated continuous bioprocessing. Despite its potential, challenges still exist in terms of its accuracy and applicability, particularly when it is used to monitor cells during stationary and decline phases. In this study, data pre-processing methods were first evaluated through cross-validation, where the first-order derivative emerged as the most effective method to diminish variability in prediction accuracy across different training datasets.
View Article and Find Full Text PDFAnal Methods
November 2017
Agricultural and Biological Engineering Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, USA.
Nitric oxide (NO) is an important signaling molecule that is involved in stress response, homeostasis, host defense, and cell development. In most cells, NO levels are in the femtomolar to micromolar range, with extracellular concentrations being much lower. Thus, real time measurement of spatiotemporal NO dynamics near the surface of living cells/tissues is a major challenge.
View Article and Find Full Text PDFCrit Rev Oncol Hematol
January 2025
Division of Medical Oncology, The Arthur G. James Comprehensive Cancer Center, Columbus, OH, USA.
Liquid biopsy (LB) has revolutionized molecular pathology, offering non-invasive insights into tumor biology. However, widespread adoption is hindered by a lack of standardized protocols, requiring robust quality control and harmonized workflows. Large-scale studies are needed to establish effective standard operating procedures (SOPs), particularly for circulating tumor DNA (ctDNA) assays tailored to different disease stages.
View Article and Find Full Text PDFEur J Pharm Biopharm
January 2025
Duquesne University Graduate School for Pharmaceutical Sciences, Pittsburgh, PA 15282, United States; Duquesne Center for Pharmaceutical Technology, Duquesne University, Pittsburgh, PA 15282, United States. Electronic address:
The adoption of pure component models, such as iterative optimization technology (IOT) algorithms, is gaining significant interest in the pharmaceutical industry, primarily because of their calibration-free/minimal calibration requirements for process analytical technology applications. The IOT methods have recently demonstrated great potential for monitoring the quality of continuous powder mixtures by Near-infrared (NIR) spectroscopy. However, the dynamic conditions of continuous manufacturing processes may limit the effectiveness of such approaches.
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