Short telomeres induce a DNA damage response, senescence, and apoptosis, thus maintaining telomere length equilibrium is essential for cell viability. Telomerase addition of telomere repeats is tightly regulated in cells. To probe pathways that regulate telomere addition, we developed the ADDIT assay to measure new telomere addition at a single telomere in vivo. Sequence analysis showed telomerase-specific addition of repeats onto a new telomere occurred in just 48 hr. Using the ADDIT assay, we found that ATM is required for addition of new repeats onto telomeres in mouse cells. Evaluation of bulk telomeres, in both human and mouse cells, showed that blocking ATM inhibited telomere elongation. Finally, the activation of ATM through the inhibition of PARP1 resulted in increased telomere elongation, supporting the central role of the ATM pathway in regulating telomere addition. Understanding this role of ATM may yield new areas for possible therapeutic intervention in telomere-mediated disease.
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http://dx.doi.org/10.1016/j.celrep.2015.10.035 | DOI Listing |
Sci Rep
December 2024
Department of Endocrinology, Metabolism, and Hypertension Research, Clinical Research Institute, NHO Kyoto Medical Center, 1-1 Mukaihata-cho, Fukakusa, Fushimi-ku, Kyoto, 612-8555, Japan.
In order to elucidate novel actions of degalactosylated whey protein (D-WP) in comparison with intact whey protein (WP), the effects of oral intake of D-WP on peripheral blood telomere length and telomerase were examined in young and aged mice. In young mice, peripheral blood telomere length was significantly elongated following oral intake of D-WP for 4 weeks. mRNA expression of both telomerase reverse transcriptase (TERT) and telomerase RNA component (TERC) was significantly increased in the peripheral blood following oral intake of D-WP for 4 weeks.
View Article and Find Full Text PDFAltern Ther Health Med
December 2024
Background: Telomere length has been identified as a marker for biological aging and stressful body states. Mind-body interventions for stress reduction such as meditation, yoga, and pranayama have been previously tested to evaluate their efficacy in restricting telomere shortening.
Primary Study Objective: In this study, the effect of Sudarshan Kriya Yoga (SKY) is investigated for its influence on telomere length.
Zhongguo Zhong Yao Za Zhi
September 2024
the First Affiliated Hospital of Henan University of Chinese Medicine Zhengzhou 450000, China.
This study explores the reparative effect of Qixiong Zuogui Compound Prescription(QXZG) intervention on the blood-brain barrier(BBB) in the aging brain with middle cerebral artery occlusion(MCAO) in rats mediated by bone marrow stem cells(BMSCs)-derived exosomes, as well as its anti-aging mechanism. An aging MCAO composite model was established using D-galactose-induced aging combined with line embolism. Rats were divided into young sham surgery group, aging sham surgery group, model group, exosome group, and exosome with traditional Chinese medicine(TCM) intervention group.
View Article and Find Full Text PDFEvolution
December 2024
School of Natural Sciences, University of Tasmania, Hobart, Tasmania 7001, Australia.
Telomeres are DNA-protein structures that primarily protect chromosomes and serve multiple functions of gene regulation. When cells divide, telomeres shorten and their main repair system in ectotherms - telomerase - replaces lost nucleotide complexes ((T2AG3)n in vertebrates). It remains a challenge to experimentally investigate resource requirements for telomere maintenance and its effects on lifespan-reproductive tradeoffs in the wild.
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