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Application of Circular Dichroism Spectroscopy to the Analysis of the Interaction Between the Estrogen Receptor Alpha and Coactivators: The Case of Calmodulin. | LitMetric

Application of Circular Dichroism Spectroscopy to the Analysis of the Interaction Between the Estrogen Receptor Alpha and Coactivators: The Case of Calmodulin.

Methods Mol Biol

Laboratoire des Biomolécules, Sorbonne Universités, UPMC Univ Paris 06, CNRS-UMR 7203, Université Pierre et Marie Curie-Ecole Normale Supérieure, Tour 23-33, 4, Place Jussieu, 75252, Paris Cedex 05, France.

Published: September 2016

AI Article Synopsis

Article Abstract

The estrogen receptor α ligand-binding domain (ERα-LBD) binds the natural hormone 17β-estradiol (E2) to induce transcription and cell proliferation. This process occurs with the contribution of protein and peptide partners (also called coactivators) that can modulate the structure of ERα, and therefore its specificity of action. As with most transcription factors, ERα exhibits a high content of α helix, making it difficult to routinely run spectroscopic studies capable of deciphering the secondary structure of the different partners under binding conditions. Ca(2+)-calmodulin, a protein also highly structured in α-helix, is a key coactivator for ERα activity. Here, we show how circular dichroism can be used to study the interaction of ERα with Ca(2+)-calmodulin. Our approach allows the determination not only of the conformational changes induced upon complex formation but also the dissociation constant (K d) of this interaction.

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Source
http://dx.doi.org/10.1007/978-1-4939-3127-9_19DOI Listing

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