The isolation of the phosphopeptide constituents from phosphoprotein digests is prerequisite to facilitate the mass spectrometric characterization of phosphorylation events. Here, we describe a chemical proteomics approach which combines solid phase derivatization of phosphoprotein digests with phosphopeptide enrichment by covalent chromatography. The use of the solid phase support for derivatization ensures for speed and completeness of reactions. The isolates proved highly suitable for mapping of the sites of phosphorylation by collisionally induced dissociation (CID). The method combines robustness with simplicity of operation using equipment available in biological laboratories, and may be readily extended to map the sites of O-glycosylation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-4939-3049-4_3 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Corrigendum to "Magnetic molecular imprinted covalent organic framework composite for the magnetic solid-phase extraction of bisphenol AF" [Journal of Chromatography A 1717 (2024) 464693].
J Chromatogr A
March 2025
Department of Pharmaceutical Analysis, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, China. Electronic address:
Magnetic cationic covalent organic framework for efficient enrichment and detection of phenolic endocrine disruptors in foodstuffs.
J Chromatogr A
February 2025
School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China. Electronic address:
Endocrine disrupting chemicals (EDCs) have received significant attention in the food field due to their potential health risks. Herein, we proposed a novel core-shell structure magnetic cationic covalent organic framework (EB-DHTA-iCOF@FeO) designed for the efficient enrichment of trace-level EDCs in foodstuffs and analyzed using HPLC-MS/MS. Due to the phenolic EDCs structure possessing hydroxyl functional groups which become protonated under alkaline conditions, resulting in the formation of negatively charged anions.
View Article and Find Full Text PDFN-doped graphene quantum dots combined with Ag nanoparticles for luminescence based analytical sensing of gentamycin after solid-phase extraction in a molecularly-imprinted polymer.
J Pharm Biomed Anal
March 2025
Department of Chemistry, Pontifical Catholic University of Rio de Janeiro (PUC-Rio), Rio de Janeiro, Brazil. Electronic address:
A luminescence-based method was developed to detect gentamicin using silver nanoparticles (AgNPs) associated with nitrogen-doped graphene quantum dots (N-GQDs). When gentamicin sulfate interacts with the AgNPs/N-GQDs system, the characteristic blue fluorescence of N-GQDs, which had been previously turned off by AgNPs, is restored. Under specific conditions (such as the amount of synthesis dispersion and pH), this AgNPs/N-GQDs probe enabled quantification of gentamicin ranging from 3.
View Article and Find Full Text PDFDegradative removal of diclofenac from wastewater - Statistical and analytical approaches to understand degradation pathways.
J Environ Manage
March 2025
Université D'Artois, IUT de Béthune, 62400, Béthune, France; LASIRE, Equipe Physico-Chimie de L'Environnement, CNRS UMR 8516, Université de Lille, Sciences et Technologies, Villeneuve D'Ascq Cedex 59655, Lille, France. Electronic address:
In environmental management area, quality of water is a major growing concern with the emerging contaminants such as pesticides and pharmaceuticals likely to be present at low concentrations in water bodies, thereby potentially harming the ecosystem and human health. Diclofenac (DCF), a commonly used drug, has been found in wastewater, surface water, and drinking water sources, whose elimination can be a challenge issue. This study investigates the elimination of DCF by the photochemical method from aqueous media using short-wavelength radiation and hydrogen peroxide.
View Article and Find Full Text PDFPhase Change-Mediated Capture of Carbon Dioxide from Air with a Molecular Triamine Network Solid.
J Am Chem Soc
March 2025
Institute for Decarbonization Materials, University of California, Berkeley, California 94720, United States.
The efficient removal of CO from exhaust streams and even directly from air is necessary to forestall climate change, lending urgency to the search for new materials that can rapidly capture CO at high capacity. The recent discovery that diamine-appended metal-organic frameworks can exhibit cooperative CO uptake via the formation of ammonium carbamate chains begs the question of whether simple organic polyamine molecules could be designed to achieve a similar switch-like behavior with even higher separation capacities. Here, we present a solid molecular triamine, 1,3,5-tris(aminomethyl)benzene (TriH), that rapidly captures large quantities of CO upon exposure to humid air to form the porous, crystalline, ammonium carbamate network solid TriH(CO)·HO (TriHCO).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!