Enzymes working in organic solvents are important for analytical chemistry, catalysis, and mechanistic studies. Although a few protein enzymes are highly active in organic solvents, little is known regarding nucleic acid-based enzymes. Herein, we report the first RNA-cleaving DNAzyme, named EtNa, that works optimally in concentrated organic solvents containing only monovalent Na(+). The EtNa DNAzyme has a rate of 2.0 h(-1) in 54% ethanol (with 120 mM NaCl and no divalent metal ions), and a Kd of 21 mm Na(+). It retains activity even in 72% ethanol as well as in DMSO. With 4 mm Na(+), the rate in 54% ethanol is >1000-fold higher than that in water. We also demonstrated the use of EtNa to measuring the ethanol content in alcoholic drinks. In total, this DNAzyme has three unique features: divalent metal independent activity, Na(+) selectivity among monovalent metals, and acceleration by organic solvents.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/cbic.201500603 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Confinement and Catalysis within Designed Peptide Barrels.
J Am Chem Soc
January 2025
School of Chemistry, University of Bristol, Cantock's Close, Bristol BS8 1TS, U.K.
protein design has advanced such that many peptide assemblies and protein structures can be generated predictably and quickly. The drive now is to bring functions to these structures, for example, small-molecule binding and catalysis. The formidable challenge of binding and orienting multiple small molecules to direct chemistry is particularly important for paving the way to new functionalities.
View Article and Find Full Text PDFA Multifunctional Synergistic Solar-Driven Interfacial Evaporator for Desalination and Photocatalytic Degradation.
ACS Appl Mater Interfaces
January 2025
State Key Laboratory of Silicate Materials for Architectures, Wuhan University of Technology, Wuhan 430070, P. R. China.
The scarcity of freshwater resources and the treatment of dye wastewater have emerged as unavoidable challenges that need to be addressed. The combination of solar-driven interfacial evaporation, photocatalytic degradation, and superhydrophobic surface provides an effective approach for seawater desalination and the treatment of organic dyes. In this study, we fabricated a multifunctional synergistic solar evaporator by depositing cupric oxide nanoparticles onto polypyrrole (PPy) coating and subsequently modified it with a hydrophobic agent successfully.
View Article and Find Full Text PDFRational Design of Porous Organic Framework (POF) for Efficient Conversion of CO2 to Cyclic Carbonates and 2-Oxazolidinones at Atmospheric Pressure Conditions.
Chem Asian J
January 2025
Indian Institute of Technology Ropar, Chemistry, Nangal Road, 140001, Rupnagar, INDIA.
Carbon dioxide (CO2) capture and its subsequent catalytic fixation into usable compounds represent a potential approach for addressing the energy problem and the implications of global warming. Hence, it is necessary to develop effective catalytic systems required for the transformation of CO2 into valuable chemicals/fuels. Herein, we rationally designed a hydroxyl-functionalized porous organic framework (OH-POF) consisting of both acidic (OH) as well as basic N sites for the transformation of CO2 using epoxides for the production of cyclic carbonates (CCs), a useful commodity chemical under environmental-friendly, metal/solvent/co-catalyst-free conditions.
View Article and Find Full Text PDFPhytochemical analysis and biological activities of solvent extracts and silver nanoparticles obtained from Woodwardia unigemmata (Makino) Nakai.
PLoS One
January 2025
Department of Pharmaceutics, College of Pharmacy, King Khalid University, Abha, Saudi Arabia.
Multidrug resistant bacteria are causing health problems and economic burden worldwide; alternative treatment options such as natural products and nanoparticles have attained great attention recently. Therefore, we aimed to determine the phytochemicals, antibacterial potential, and anticancer activity of W. unigemmata.
View Article and Find Full Text PDFExcited-State Rotational Dynamics of Amine-Functionalized Terephthalic Acid Derivatives as Linker Models for Metal-Organic Frameworks.
J Phys Chem A
January 2025
Advanced Membranes and Porous Materials Center, Division of Physical Science and Engineering, King Abdullah University of Science and Technology, Thuwal 23955-6900, Kingdom of Saudi Arabia.
Understanding how structural modifications affect the photophysics of organic linkers is crucial for their integration into metal-organic frameworks (MOFs) for light-driven applications. This study explores the impact of varying the amine functional group position on two terephthalic acid derivatives─linker and linker ─by investigating their photophysics through a combination of steady-state and ultrafast laser spectroscopy and time-dependent density functional theory (TD-DFT) calculations. With tetrahydrofuran as the solvent, time-correlated single-photon counting revealed a 2-fold increase in the S excited-state lifetime of the molecule with the amine group at the meta position compared with that of the molecule with the amine group at the ortho position.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!