Recent studies have demonstrated that micro (mi)RNA molecules can be detected in the circulation and can serve as potential biomarkers of various diseases. This study used microarray analysis to identify aberrantly expressed circulating miRNAs in patients with type 1 autoimmune hepatitis (AIH) compared with healthy controls. Patients with well-documented and untreated AIH were selected from the National Hospital Organization (NHO)-AIH-liver-network database. They underwent blood sampling and liver biopsy with inflammation grading and fibrosis staging before receiving treatment. To further confirm the microarray data, circulating expression levels of miR-21 and miR-122 were quantified by real-time quantitative polymerase chain reaction in 46 AIH patients, 40 patients with chronic hepatitis C (CHC), and 13 healthy controls. Consistent with the microarray data, serum levels of miR-21 were significantly elevated in AIH patients compared with CHC patients and healthy controls. miR-21 and miR-122 serum levels correlated with alanine aminotransferase levels. Circulating levels of miR-21 and miR-122 were significantly reduced in AIH patients with liver cirrhosis, and were inversely correlated with increased stages of fibrosis. By contrast, levels of circulating miR-21 showed a significant correlation with the histological grades of inflammation in AIH. We postulate that aberrantly expressed serum miRNAs are potential biomarkers of AIH and could be implicated in AIH pathogenesis. Alternations of miR-21 and miR-122 serum levels could reflect their putative roles in the mediation of inflammatory processes in AIH.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4648542 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0136908 | PLOS |
Publication Analysis
Top Keywords
Similar Publications
Dual DNAzyme amplification-based colorimetric sensing assay for the identification and quantification of tumor-associated miRNAs.
Talanta
December 2024
State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China. Electronic address:
Herein, we present a colorimetric sensing strategy for the identification and quantification of tumor-associated miRNAs based on dual DNAzyme amplification. In this sensing ensemble, the substrate portion of the Pb-dependent 8-17 DNAzyme combines with the G-quadruplex portion to form a hairpin substrate strand. The two split 8-17 DNAzyme strands are partially complementary to the substrate strand and serve as a recognition unit for binding the target miRNA.
View Article and Find Full Text PDFDifferent expression patterns of inflammation-related genes and serum microRNAs in young-onset ischemic stroke.
Sci Rep
October 2024
Department of Neurology and Neurosurgery, University of Tartu, L. Puusepa 8, 50406, Tartu, Estonia.
Article Synopsis
- * Researchers collected blood samples from 73 patients and analyzed the expression of specific genes and miRNAs using real-time quantitative PCR, revealing significant differences in gene expression patterns between the two types of stroke.
- * Findings showed that certain inflammatory genes were more upregulated during the acute phase of cryptogenic stroke compared to LAA stroke, suggesting differing inflammatory responses associated with these two stroke types.
A combined model of six serum microRNAs as diagnostic markers for hepatocellular carcinoma.
Clin Chim Acta
January 2025
Qingdao Ruiside Medical Laboratory Co., LTD, Qingdao, Shandong 266111, PR China. Electronic address:
Background: Hepatocellular carcinoma (HCC) is associated with high morbidity and mortality, and its poor prognosis is mainly due to the lack of an effective means of early diagnosis. This study aimed to identify a group of serum microRNAs (miRNAs) as potential biomarkers for the diagnosis of HCC.
Methods: We collected 190 HCC cases, 109 benign lesions of the liver, 40 cases of non-HCC tumors, and 130 healthy controls.
The role of circulating miRNAs in the diagnosis of osteoporosis miRNAs in osteoporosis.
Rev Assoc Med Bras (1992)
September 2024
Mersin University, Medical Faculty, Department of Medical Biochemistry - Mersin, Turkey.
Objective: Osteoporosis, defined as a systemic skeletal disease, is characterized by increased bone fragility and fracture risk. Studies have shown that dysregulation of the functions of miRNAs or the mechanisms they mediate may be an important pathological factor in bone degeneration. Therefore, the aim of the study was to determine the role of miRNAs, which are thought to play a role in bone metabolism, in osteoporosis.
View Article and Find Full Text PDFAn electrochemical biosensor equipped with a logic circuit as a smart automaton for two-miRNA pattern detection.
Analyst
October 2024
College of Chemistry and Chemical Engineering, Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, China West Normal University, Nanchong, Sichuan 637002, China.
Detecting multiple targets in complex cellular and biological environments yields more reliable results than single-label assays. Here, we introduced an electrochemical biosensor equipped with computing functions, acting as a smart automaton to enable computing-based detection. By defining the logic combinations of miR-21 and miR-122 as detection patterns, we proposed the corresponding AND and OR detection automata.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!