Trehalose is a reducing disaccharide acting as a protectant against environmental stresses in many organisms. In fungi, Trehalose-6-phosphate synthase 1 (TPS1) plays a key role in the biosynthesis of trehalose. In this study, a full-length cDNA from Rhizopus oryzae encoding TPS1 (designated as RoTPS1) was isolated. The RoTPS1 cDNA is composed of 2505 nucleotides and encodes a protein of 834 amino acids with a molecular mass of 97.8 kDa. The amino acid sequence of RoTPS1 has a relatively high homology with the TPS1s in several other filamentous fungi. RoTPS1 was cloned into Saccharomyces cerevisiae and secretively expressed.
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http://dx.doi.org/10.1002/jobm.201500425 | DOI Listing |
Insects
December 2024
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China.
Due to the intensification of human activities, the ecosystems are being polluted by heavy metals. The pollution of heavy metals in agricultural systems has become a serious issue of global concern. This study detected the bioaccumulation of cadmium (Cd) in broad beans and aphids through continuous exposure to varying concentrations of Cd pollution (0, 3.
View Article and Find Full Text PDFJ Adv Res
December 2024
College of Forestry and Grassland Science, Jilin Provincial Key Laboratory of Tree and Grass Genetics and Breeding, Jilin Agriculture University, Changchun 130118, China. Electronic address:
Background: Trehalose is a nonreducing disaccharide containing two glucose molecules linked through an α,α-1,1-glycosidic bond. This unique chemical structure causes trehalose levels to fluctuate significantly in plants under stress, where it functions as an osmoprotectant, enhancing plant resistance to stress. Previous studies have confirmed that the trehalose synthesis pathway is widely conserved across most plants.
View Article and Find Full Text PDFPlant Physiol
December 2024
Guangdong Litchi Engineering Research Center, College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
Litchi (Litchi chinensis), a prominent fruit tree in the Sapindaceae, initiates flowering in response to low autumn and winter temperatures. This study investigates the epigenetic regulation of this process, focusing on the marks histone H3 lysine 27 trimethylation (H3K27me3) and its deposition genes during the chilling-induced floral induction (FId) and initiation stages. Our genomic analysis delineated the H3K27me3 deposition landscape across the prefloral induction (PFId), FId, and floral initiation (FIn) stages.
View Article and Find Full Text PDFMicrobiome
November 2024
CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China.
Background: Vestimentiferan tubeworms are deep-sea colonizers, in which chemoautotrophic symbiosis was first observed. These animals are gutless and depend on endosymbiotic bacteria for organic compound synthesis and nutrition supply. Taxonomically, vestimentiferans belong to Siboglinidae and Annelida.
View Article and Find Full Text PDFPlant Physiol
December 2024
Max Planck Institute of Molecular Plant Physiology, Department Metabolic Networks, 14476 Potsdam, Germany.
The timing of flowering in plants is modulated by both carbon (C) and nitrogen (N) signaling pathways. In a previous study, we established a pivotal role of the sucrose-signaling trehalose 6-phosphate pathway in regulating flowering under N-limited short-day conditions. In this work, we show that both wild-type Arabidopsis (Arabidopsis thaliana) plants grown under N-limited conditions and knock-down plants of TREHALOSE PHOSPHATE SYNTHASE 1 induce FLOWERING LOCUS C (FLC) expression, a well-known floral repressor associated with vernalization.
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