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Development of Novel Squid Gladius Biomaterials for Cornea Tissue Engineering.
Mar Drugs
November 2024
Tissue Engineering Group, Department of Histology, Faculty of Medicine, University of Granada, E18016 Granada, Spain.
Cornea tissue engineering is strictly dependent on the development of biomaterials that fulfill the strict biocompatibility, biomechanical, and optical requirements of this organ. In this work, we generated novel biomaterials from the squid gladius (SG), and their application in cornea tissue engineering was evaluated. Results revealed that the native SG (N-SG) was biocompatible in laboratory animals, although a local inflammatory reaction was driven by the material.
View Article and Find Full Text PDFSilver mean sequence in extended depth of focus intraocular lenses: a comparative study of kinoform and stepwise designs.
Biomed Opt Express
December 2024
Departamento de Óptica y Optometría y Ciencias de la Visión, Universitat de València, Burjassot, Spain.
In this work, we present two new multifocal intraocular lens (MIOL) designs, both based on the silver mean kinoform diffractive lens. We demonstrate that a single aperiodic diffractive profile can be used to create two different MIOLs: one with a kinoform structure and the other with a stepwise profile. Quantitative assessment of the designs was carried out using the through focus modulation transfer function and the area under the modulation transfer function for the prediction of their visual performance.
View Article and Find Full Text PDFSimultaneous 2-photon and 3-photon excitation with a red fluorescent protein-cyanine dye probe pair in the 1700-nm excitation window for deep neurovascular imaging.
Biomed Opt Express
December 2024
Guangdong Provincial Key Laboratory of Biomedical Optical Imaging Technology, Center for Biomedical Optics and Molecular Imaging, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
imaging of the neurovascular network is considered to be one of the most powerful approaches for understanding brain functionality. Nevertheless, simultaneously imaging the biological neural network and blood vessels in deep brain layers in a non-invasive manner remains to a major challenge due to the lack of appropriate labeling fluorescence probe pairs. Herein, we proposed a 2-photon and 3-photon fluorescence probe pair for neurovascular imaging.
View Article and Find Full Text PDFIdentifying retinal pigment epithelium cells in adaptive optics-optical coherence tomography images with partial annotations and superhuman accuracy.
Biomed Opt Express
December 2024
Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
Retinal pigment epithelium (RPE) cells are essential for normal retinal function. Morphological defects in these cells are associated with a number of retinal neurodegenerative diseases. Owing to the cellular resolution and depth-sectioning capabilities, individual RPE cells can be visualized in vivo with adaptive optics-optical coherence tomography (AO-OCT).
View Article and Find Full Text PDFIn vivo dual-plane 3-photon microscopy: spanning the depth of the mouse neocortex.
Biomed Opt Express
December 2024
The Sainsbury Wellcome Centre for Circuits and Behaviour, University College London, 25 Howland Street, W1T 4JG, London, United Kingdom.
Cortical computations arise from patterns of neuronal activity that span across all cortical layers and cell types. Three-photon excitation has extended the depth limit of imaging within the mouse brain to encompass all cortical layers. However, simultaneous three-photon imaging throughout cortical layers has yet to be demonstrated.
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