A simple device and associated analytical methods are reported. We provide objective and accurate determination of saliva alcohol concentrations using smartphone-based colorimetric imaging. The device utilizes any smartphone with a miniature attachment that positions the sample and provides constant illumination for sample imaging. Analyses of histograms based on channel imaging of red-green-blue (RGB) and hue-saturation-value (HSV) color space provide unambiguous determination of blood alcohol concentration from color changes on sample pads. A smartphone-based sample analysis by colorimetry was developed and tested with blind samples that matched with the training sets. This technology can be adapted to any smartphone and used to conduct color change assays.
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http://dx.doi.org/10.1364/AO.54.009183 | DOI Listing |
Anal Methods
January 2025
Department of Chemistry, Karpagam Academy of Higher Education, Coimbatore, Tamil Nadu, India.
A new rhodamine based turn on florescent probe ()-3',6'-bis(ethylamino)-2-(((6-methoxy-2-oxo-1,2-dihydroquinolin-3-yl)methylene)amino)-2',7'-dimethylspiro[isoindoline-1,9'-xanthen]-3-one (RME) was efficiently synthesized through a simple condensation reaction of 2-amino-3',6'-bis(ethylamino)-2',7'-dimethylspiro[isoindoline-1,9'-xanthen]-3-one and 6-methoxy-2-oxo-1,2-dihydroquinoline-3-carbaldehyde. The receptor RME is highly non-fluorescent and when copper ions (Cu ions) are added in DMF/water (1 : 2, v/v) medium, the receptor RME exhibits a specific "turn-on" colorimetric and fluorometric response. Moreover, RME binding with Cu ions produced a remarkable color variation that was perceptible to the human eye, changing from colorless to pink.
View Article and Find Full Text PDFACS Appl Mater Interfaces
January 2025
Key Laboratory for Special Functional Aggregate Materials of Education Ministry, School of Chemistry and Chemical Engineering, Shandong University, Jinan, Shandong 250100, China.
The adsorption of DNA probes onto nanomaterials represents a promising bioassay technique, generally employing fluorescence or catalytic activity to generate signals. A significant challenge is maintaining the catalytic activity of chromogenic catalysts during detection while enhancing accuracy by overcoming the limitations of single-signal transmission. This article presents an innovative multimodal analysis approach that synergistically combines the oxidase-like activity of Fe-N-C nanozyme (Fe-NC) with red fluorescent carbon quantum dots (R-CQDs), further advancing the dual-mode analysis method utilizing R-CQDs@Fe-NC.
View Article and Find Full Text PDFBiosens Bioelectron
December 2024
Biophotonic Nanosensors Laboratory, Centro de Física Aplicada y Tecnología Avanzada (CFATA), Universidad Nacional Autónoma de México (UNAM), Querétaro, 76230, Mexico. Electronic address:
Smartphone-based colorimetric (bio)sensing is a promising alternative to conventional detection equipment for on-site testing, but it is often limited by sensitivity to lighting conditions. These issues are usually avoided using housings with fixed light sources, increasing the cost and complexity of the on-site test, where simplicity, portability, and affordability are a priority. In this study, we demonstrate that careful optimization of color space can significantly boost the performance of smartphone-based colorimetric sensing, enabling housing-free, illumination-invariant detection.
View Article and Find Full Text PDFAnal Chim Acta
January 2025
Hunan Provincial Key Laboratory of Cytochemistry, School of Chemistry and Chemical Engineering, Changsha University of Science and Technology, Changsha, 410114, PR China.
Alkaline phosphatase (ALP) is a critical biomarker associated with various physiological and pathological processes, making its detection essential for disease diagnosis and biomedical research. In this study, we developed a novel, simple, and portable visual quantification method for ALP activity in cells using an efficient CuZnS nanomaterial with peroxidase-like properties, integrated into a smartphone-based platform for enhanced usability. The CuZnS nanomaterial catalyzes the breakdown of H₂O₂, generating ·OH radicals that oxidize the colorless substrate TMB into blue oxTMB, which is subsequently reduced back to TMB by ascorbic acid (AA).
View Article and Find Full Text PDFAnal Chim Acta
January 2025
College of Resources and Environment, Southwest University, Chongqing, 400716, China; Key Laboratory of Biomedical Analytics (Southwest University), Chongqing Science and Technology Bureau, College of Pharmaceutical Sciences, Southwest University, Chongqing, 400715, China. Electronic address:
Background: Because arsenate (As(V)) is a highly toxic pollutant, timely on-site monitoring of its concentration is crucial for mitigating potential environmental and health hazards. Traditional on-site detection methods for As(V) often face limitations of long response time and low sensitivity. Nanozymes are nanomaterials that exhibit enzyme-like catalytic activity.
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