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| http://dx.doi.org/10.1021/acs.analchem.5b04139 | DOI Listing |
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Proteomics-driven discovery of LCAT as a novel biomarker for liver metastasis in colorectal cancer.
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Department of General Surgery, Affiliated Zhongshan Hospital of Dalian University, No. 6 Jiefang Street, Zhongshan District, Dalian, 116001, Liaoning Province, China.
Background: This study aimed to identify molecular markers that influence liver metastasis in colorectal cancer (CRC) and assess their clinical relevance.
Methods: Proteomic analysis compared differential protein expression between CRC patients with liver metastasis (CRLM) and those without (CRNLM). Bioinformatics and survival analyses identified key proteins and validated them using the TCGA database for expression and clinical significance.
Microfluidic magnetic droplet-based chemiluminescence enzyme immunoassay for multiplex sepsis biomarker screening.
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Department of Neurological Rehabilitation, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325027, P.R. China.
Sepsis is a systemic inflammatory response syndrome caused by infection, requiring the joint detection of multiplex biomarkers for specific diagnosis. Here, we present a chemiluminescence enzyme immunoassay based on microfluidic magnetic droplets for multiplex sepsis biomarker screening. The droplet-based chemiluminescence enzyme immunoassay (CLIA) technology utilizes multicolor-encoded microspheres to distinguish biomarkers and mesoporous silica-loaded enzymes for signal amplification and catalytic fluorescent substrates.
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Center for Engineering in Medicine and Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, United States.
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Bimetallic heterostructures, including core-shell and Janus configurations, often offer unique electrocatalytic properties compared to monometallic nanoparticles. However, achieving precise control over both elemental composition and spatial arrangement within these structures remains a challenge. Here, an electrosynthesis method is introduced that enables the fabrication of heterostructured bimetallic nanoparticles with precise, independent control of their elemental distribution.
View Article and Find Full Text PDFSingle extracellular vesicle detection assay identifies membrane-associated α-synuclein as an early-stage biomarker in Parkinson's disease.
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Nuffield Department of Clinical Neurosciences & Kavli Institute for Nanoscience Discovery, University of Oxford, Oxford, UK. Electronic address:
Accurate diagnosis of early Parkinson's disease requires platforms suitable for detecting minute amounts of neuronally derived biomarkers in the massive protein excess of easily accessible biofluids such as blood. Here, we describe an on-chip droplet-confined fluorescence reporting assay that identified α-synuclein on the membrane of L1CAM+ extracellular vesicles (EVs) immunocaptured from human serum and corroborate this finding by super-resolution direct stochastic optical reconstruction microscopy (dSTORM) microscopy. Using conditioned media from neuroblastoma cells expressing α-synuclein mutants or patient-derived induced pluripotent stem cell (iPSC) neurons with α-synuclein gene triplication, we found that association of α-synuclein with the L1CAM+ EV surface is increased under pathological conditions.
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